uva Reticulata海藻水解过程的优化与HNO3溶剂生产生物乙醇

Sefrinus Maria Dolfi Kolo
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引用次数: 0

摘要

制造生物燃料和替代石油基燃料的一种替代方法是将网状海藻中的非食品成分转化为生物乙醇。70%的地球表面覆盖着微藻和海藻,它们可以转化为生物乙醇。Ulva海藻含有50.3%的碳水化合物,以异多糖的形式存在,如葡萄糖、阿拉伯糖、鼠李糖和木糖。通过不同酸浓度(1、3、5、7%)、水解时间(30、40、50、60分钟)和水解温度(75、100、125、150℃)对微波辐照下HNO3催化海藻水解进行了优化。在温度为30°C, pH为4.5的条件下,通过不同的接种浓度(6,8和10% (v/v))进行发酵,发酵5,6和7天。利用扫描电镜(SEM)对样品的表面织构进行了分析。采用二硝基水杨酸(DNS)法测定还原糖浓度。乙醇分析采用气相色谱法(GC)。SEM分析结果表明,在水解前,粉末的表面形貌仍然致密完整。而经HNO3水解后,粉末的表面结构受到明显的破坏。结果表明,预处理青豆粉的最佳条件为酸浓度为7%,反应时间为50 min,反应温度为150℃,反应功率为250瓦,可得到还原糖浓度为86.5 g/L的水解产物。用酵母对水解液进行发酵,产生物乙醇浓度为37.2%,采用气相色谱仪分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimasi Proses Hidrolisis Rumput Laut Ulva Reticulata dengan Pelarut HNO3 untuk Produksi Bioetanol
One alternative to make biofuels and replace petroleum-based fuels is to convert non-food ingredients from Ulva reticulata seaweed into bioethanol. Seventy percent of the earth's surface is covered by microalgae and seaweeds that can be converted into bioethanol. Ulva seaweed contains 50.3% carbohydrates in the form of heteropolysaccharides  such as glucose, arabinose, rhamnose and xylose. Optimization of the seaweed hydrolysis catalyzed by HNO3 using Microwave irradiation was done by varying acid concentration (1, 3, 5, 7%), hydrolysis time (30, 40, 50, and 60 minutes), and hydrolysis temperature (75, 100, 125, 150°C). Fermentation was carried out by varying inoculum concentrations (6, 8, and 10% (v/v)) for 5, 6 and 7 days at a temperature of 30°C and a pH of 4.5. Analysis of the surface texture of the sample was carried out by Scanning Electron Microscopy (SEM). The analysis of reducing sugars concentration was carried out using the dinitrosalicylate (DNS) method. Ethanol analysis was carried out by Gas Chromatography (GC). The results of SEM analysis showed that prior to hydrolysis, the surface morphology of the powder was still compact and intact. Whereas after being hydrolyzed with HNO3 it was seen that the surface texture of the powder suffered significant damage. The hydrolysis results showed that the optimum conditions during the pretreatment of U. reticulata powder was at acid concentration of 7%, reaction time of 50 minutes, reaction temperature of 150°C and 250 watts of power which gave hydrolysate with reducing sugar concentration of 86.5 g/L. Fermentation of the hydrolysate using yeast Saccharomyces cerevisiae produced bioethanol with concentration of 37.2% as analyzed using a gas chromatograph.
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