利用预测序参量和15N核磁共振弛豫分析球状蛋白的动力学性质

IF 0.4 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of the Korean magnetic resonance society Pub Date : 2017-03-20 DOI:10.6564/JKMRS.2017.21.1.026

Jong-Jae Yi, Won-Je Kim, J. Rhee, Jongsoo Lim, Bong‐Jin Lee, W. Son

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引用次数: 0

摘要

蛋白质的动态特性可以提供蛋白质-配体和蛋白质-蛋白质相互作用的关键信息。尽管它们很有用，但由于蛋白质的稳定性和短期测量，蛋白质动力学的性质并不容易获得。本文提出了一种综合分析动力特性的方法。它利用了预测的序参量和核磁共振弛豫数据，如t1、t2和异核NOE。该方法可以通过无模型分析，在不需要精确的序参数等动力学参数的情况下，粗略地了解蛋白质的柔韧性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Elucidating the Dynamic Properties of Globular Protein using Predicted Order Parameters and 15N NMR Relaxation

Dynamic properties of proteins can present key information on protein-ligand and protein-protein interaction. Despite their usefulness, the properties of protein dynamics have not been obtained easily due to protein stability and short-term measurement. Here, it is shown that combined method for analysis of dynamical properties. It utilizes predicted order parameter and NMR relaxation data such as T 1 , T 2 , and heteronuclear NOE. The suggested method could be used to know the flexibility of protein roughly without precise dynamical parameters such as order parameters through model-free analysis.

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来源期刊

Journal of the Korean magnetic resonance society BIOCHEMICAL RESEARCH METHODS-

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66.70%

发文量