微波辅助柠檬酸盐提取（MaCE）作为高压釜柠檬酸盐萃取（ACE）土壤蛋白质组分的替代方案

IF 1.5 4区农林科学 Q4 SOIL SCIENCE

Canadian Journal of Soil Science Pub Date : 2023-07-21 DOI:10.1139/cjss-2023-0016

Qianyi Wu, K. Congreves, R. Farrell

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引用次数: 0

摘要

土壤蛋白质是土壤健康的重要指标，对于土壤健康评估，通常使用高压灭菌柠檬酸盐提取（ACE）进行测定，然后使用Bradford或二辛可宁酸（BCA）测定进行蛋白质定量。在这里，我们研究了一种使用微波辅助柠檬酸盐提取（MaCE）的替代提取工艺。我们表明，蛋白质产量随着提取时间的增加而增加，但在提取时间短至15分钟的情况下，可以获得与使用标准ACE方法获得的产量相当的产量。据我们所知，这是第一份使用微波辅助提取来确定土壤蛋白质库的报告。

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Microwave-assisted citrate extraction (MaCE) as an alternative to autoclave citrate extraction (ACE) of a soil protein fraction

Soil protein is an important indicator of soil health and for soil health assessments is usually determined using autoclaved citrate extraction (ACE) followed by protein quantification using the Bradford or bicinchoninic acid (BCA) assay. Here we investigated an alternative extraction process using microwave-assisted citrate extraction (MaCE). We show that protein yield increases as the extraction time increases, but that yields comparable to those obtained using the standard ACE method can be obtained with an extraction time as short as 15 min. To the best of our knowledge, this is the first report of microwave-assisted extraction being used to determine this soil protein pool.

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来源期刊

Canadian Journal of Soil Science 农林科学-土壤科学

CiteScore

2.90

自引率

11.80%

发文量

审稿时长

6.0 months

期刊介绍： The Canadian Journal of Soil Science is an international peer-reviewed journal published in cooperation with the Canadian Society of Soil Science. The journal publishes original research on the use, management, structure and development of soils and draws from the disciplines of soil science, agrometeorology, ecology, agricultural engineering, environmental science, hydrology, forestry, geology, geography and climatology. Research is published in a number of topic sections including: agrometeorology; ecology, biological processes and plant interactions; composition and chemical processes; physical processes and interfaces; genesis, landscape processes and relationships; contamination and environmental stewardship; and management for agricultural, forestry and urban uses.