萎缩木霉:一个来自森林环境的具有高抗菌潜力的次级代谢产物的分离物

IF 0.9 Q4 CHEMISTRY, MULTIDISCIPLINARY
J. Víglaš, P. Olejníková
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引用次数: 2

摘要

摘要这项工作的重点是在Zázrivá村(斯洛伐克北部,奥拉瓦地区)周围的森林中发现的新分离的萎缩性木霉O1的特征。通过对其rDNA的内部转录间隔区(its)进行测序来鉴定该分离物。T.atroviride O1刺激了模式植物Lepidium sativum侧根的发育。同时,该分离株已证明其具有很高的真菌寄生潜力,因为它显示出攻击植物病原真菌(Alternaria alternata、Fusarium tumorum、Botrytis cinerea)菌落的能力。该分离物产生次级代谢产物,分离并测试其对革兰氏阳性菌表皮葡萄球菌和金黄色葡萄球菌的抗菌活性。这些细菌的生长分别被抑制到10%和40%。对两种念珠菌的生长也有很强的抑制作用(10%的生长)。然而,三种植物病原真菌(Alternaria alternata、Botrytis cinerea和Fusarium culmorum)的生长参数受到的影响较小(与对照相比生长75%)。尝试鉴定从T.atroviride O1中分离的次级代谢产物。通过MALDI-TOF质谱法检测到已知的蛋白胨,20-21个氨基酸长,但也有较短的肽。因此,本研究证明了分离物T.atroviride O1的植物生长促进作用、强大的分枝寄生潜力和抗菌活性,这在一定程度上可归因于次级代谢产物的产生。这种分离物在生态农业的生物防治中确实具有潜力。需要进一步研究,特别是鉴定产生的次级代谢产物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Trichoderma atroviride: an isolate from forest environment with secondary metabolites with high antimicrobial potential
Abstract This work was focused on the characterization of novel isolate of Trichoderma atroviride O1, found in the forest around the village of Zázrivá (the Northern Slovakia, region Orava). The isolate was identified by sequencing its internal transcribed spacer (ITS) region of rDNA. T. atroviride O1 stimulated the development of lateral roots of model plant Lepidium sativum. Simultaneously, the isolate has proved its high mycoparasitic potential as it displayed the ability to attack colonies of phytopathogenic fungi (Alternaria alternata, Fusarium culmorum, Botrytis cinerea). This isolate produced secondary metabolites, which were isolated and tested for the antimicrobial activity against gram-positive bacteria Staphylococcus epidermidis and Staphylococcus aureus. The growth of these bacteria was suppressed to 10 % and 40 %, respectively. The suppression of the growth of two Candida species was also strong (10 % growth). However, growth parameters of three phytopathogenic fungi (Alternaria alternata, Botrytis cinerea and Fusarium culmorum) were less affected (75 % growth in comparison with the control). Attempts were made to characterize secondary metabolites isolated from T. atroviride O1. Known peptaibols, 20—21 amino acid long, but also shorter peptides, were detected by MALDI-TOF mass spectrometry. Thus, this study demonstrates the plant growth promotion, strong mycoparasitic potential and antimicrobial activity of the isolate T. atroviride O1, which could be in part ascribed to the production of secondary metabolites. This isolate does have a potential in the biocontrol in eco-farming. Further study, particularly, the identification of produced secondary metabolites, is needed.
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来源期刊
Acta Chimica Slovaca
Acta Chimica Slovaca CHEMISTRY, MULTIDISCIPLINARY-
自引率
12.50%
发文量
11
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