白背飞虱的染色体水平基因组组装和性别特异性差异转录组

IF 1.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Genomics Pub Date : 2023-01-02 DOI:10.2174/1389202924666230102092822

C. Zhang, Yu-Xuan Ye, Dan-Ting Li, Si-Yu Zhang, Z. Shen

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引用次数: 0

摘要

药物化学中的抗炎和变态反应剂在这项研究中，我们报告了使用第三代测序技术、Hi-C数据和全长转录本对染色体水平的WBPH基因组与特征性染色体的从头组装，并提供了性别特异性转录组的密密景观。我们产生了具有2.20Mb的重叠群N50和45.25Mb的支架N50的高质量染色体水平组装。鉴定出14条常染色体和1条X染色体。99.5%以上的组装碱基位于15条染色体上。在最终组装中检测到95.9%的BUSCO完整基因，并注释了16880个基因。基因组完整、性染色体明确、转录组图谱全面、RNA干扰效率高、生命周期短，使WBPH成为功能基因组学的有效研究对象。

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Chromosome-level genome assembly and sex-specific differential transcriptome of the white-backed planthopper, Sogatella furcifera

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry In this study, we report a de novo assembly of the chromosome-level WBPH genome with characterized sex chromosomes using third-generation sequencing technologies, Hi-C data and full-length transcripts, and provide a dense landscape of sex-specific transcriptome. We generated a high-quality chromosome-level assembly with a contig N50 of 2.20 Mb and a scaffold N50 of 45.25 Mb. Fourteen autosomes and one X chromosomes were identified. More than 99.5% of the assembled bases located on the 15 chromosomes. 95.9% of the BUSCO complete genes were detected in the final assembly and 16,880 genes were annotated. The integrated genome, definite sex chromosomes, comprehensive transcriptome profiles, high efficiency of RNA interference and short life cycle substantially made WBPH an efficient research object for functional genomics.

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来源期刊

Current Genomics 生物-生化与分子生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Current Genomics is a peer-reviewed journal that provides essential reading about the latest and most important developments in genome science and related fields of research. Systems biology, systems modeling, machine learning, network inference, bioinformatics, computational biology, epigenetics, single cell genomics, extracellular vesicles, quantitative biology, and synthetic biology for the study of evolution, development, maintenance, aging and that of human health, human diseases, clinical genomics and precision medicine are topics of particular interest. The journal covers plant genomics. The journal will not consider articles dealing with breeding and livestock. Current Genomics publishes three types of articles including: i) Research papers from internationally-recognized experts reporting on new and original data generated at the genome scale level. Position papers dealing with new or challenging methodological approaches, whether experimental or mathematical, are greatly welcome in this section. ii) Authoritative and comprehensive full-length or mini reviews from widely recognized experts, covering the latest developments in genome science and related fields of research such as systems biology, statistics and machine learning, quantitative biology, and precision medicine. Proposals for mini-hot topics (2-3 review papers) and full hot topics (6-8 review papers) guest edited by internationally-recognized experts are welcome in this section. Hot topic proposals should not contain original data and they should contain articles originating from at least 2 different countries. iii) Opinion papers from internationally recognized experts addressing contemporary questions and issues in the field of genome science and systems biology and basic and clinical research practices.