白背飞虱的染色体水平基因组组装和性别特异性差异转录组

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-02 DOI:10.2174/1389202924666230102092822

C. Zhang, Yu-Xuan Ye, Dan-Ting Li, Si-Yu Zhang, Z. Shen

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引用次数: 0

摘要

药物化学中的抗炎和变态反应剂在这项研究中，我们报告了使用第三代测序技术、Hi-C数据和全长转录本对染色体水平的WBPH基因组与特征性染色体的从头组装，并提供了性别特异性转录组的密密景观。我们产生了具有2.20Mb的重叠群N50和45.25Mb的支架N50的高质量染色体水平组装。鉴定出14条常染色体和1条X染色体。99.5%以上的组装碱基位于15条染色体上。在最终组装中检测到95.9%的BUSCO完整基因，并注释了16880个基因。基因组完整、性染色体明确、转录组图谱全面、RNA干扰效率高、生命周期短，使WBPH成为功能基因组学的有效研究对象。

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Chromosome-level genome assembly and sex-specific differential transcriptome of the white-backed planthopper, Sogatella furcifera

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry In this study, we report a de novo assembly of the chromosome-level WBPH genome with characterized sex chromosomes using third-generation sequencing technologies, Hi-C data and full-length transcripts, and provide a dense landscape of sex-specific transcriptome. We generated a high-quality chromosome-level assembly with a contig N50 of 2.20 Mb and a scaffold N50 of 45.25 Mb. Fourteen autosomes and one X chromosomes were identified. More than 99.5% of the assembled bases located on the 15 chromosomes. 95.9% of the BUSCO complete genes were detected in the final assembly and 16,880 genes were annotated. The integrated genome, definite sex chromosomes, comprehensive transcriptome profiles, high efficiency of RNA interference and short life cycle substantially made WBPH an efficient research object for functional genomics.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.