从凋亡和自噬标记物的变化评价犬自然感染犬瘟热病毒后脑细胞凋亡和自吞噬活性

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria-Beograd Pub Date : 2022-06-01 DOI:10.2478/acve-2022-0016

Ş. Değirmençay, Selçuk Özdemir

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引用次数: 0

摘要

摘要本研究通过实时荧光定量PCR检测急性神经症状犬CDV感染脑组织中cas3、cas8、cas9、Bax、Bcl-2、LC3B和Beclin-1的表达，探讨细胞凋亡和自噬的激活情况。与对照组相比，自噬标志物Beclin-1和LC3B的表达水平显著上调(p < 0.001)。凋亡标志物cas3、cas8、cas9、Bax的表达量与对照组相比略有上调，Bcl-2的表达量略有下调(p < 0.05)。因此，在有急性神经症状的狗中，自噬标记物比凋亡标记物更活跃。综上所述，自噬参与了犬瘟热脱髓鞘的发病机制。了解这一点可能有助于制定新的治疗策略，例如新的有效抗病毒药物。

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Evaluation of Apoptosis and Autophagy Activities in the Brain of Dogs Naturally Infected with Canine Distemper Virus Based on Changes in Apoptotic and Autophagic Markers

Abstract This study investig ated the activation of apoptosis and autophagy in CDV infected brain tissues of dogs with acute neurological signs, by determining Cas-3, Cas-8, Cas-9, Bax, Bcl-2, LC3B, and Beclin-1 expression with real-time PCR. The expression levels of Beclin-1 and LC3B, autophagy markers, were significantly up-regulated in comparison with the control group (p < 0.001). The expression levels of apoptotic markers Cas-3, Cas-8, Cas-9 and Bax were slightly up-regulated, but Bcl-2 was slightly down-regulated in contrast to the control group (p < 0.05). Therefore, the autophagy markers were more activated than apoptotic markers in dogs with acute neurological signs. In conclusion, autophagy takes part in the pathogenesis of demyelination in canine distemper. Knowing this may be helpful to create new therapeutic strategies, such as new effective antiviral medicines.

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来源期刊

Acta Veterinaria-Beograd 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

18-36 weeks

期刊介绍： The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.