实验性过敏性脑脊髓炎的建模和形成方法Evaluation

Q3 Pharmacology, Toxicology and Pharmaceutics
O. Nefodov, I. Belenichev, M. Fedchenko, O. Popazova, V. Ryzhenko, O. Morozova
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引用次数: 0

摘要

引言:实验性自身免疫性(变态反应性)脑脊髓炎(EAE)是指用弗氏刺激器皮内注射大脑、脊髓和外周神经匀浆诱发的一种真正的神经系统自身免疫性疾病。材料与方法:采用白色非线性大鼠进行实验研究。为了诱导实验性变态反应性脑脊髓炎(EAE),使用同源脑匀浆,这在其他药物(同源、异质性脑和脊髓匀浆)中领先于脑炎。将每100g体重0.1ml的致脑炎悬浮液的混合物注射到动物尾部基底的结缔组织中。结果与讨论:根据结果,大鼠体重减轻,平均10-12天出现神经系统异常症状。我们对EAE形成的实验研究通过电子显微镜在形态学上得到了证实。结论:因此,该技术的使用使我们能够获得实验性变态反应性脑脊髓炎形式的多发性硬化症的模拟病理状况,并可用于未来的研究,以确定在诱导实验性病理状况时身体免疫和神经系统变化的适当规律、程度和性质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of methods of modeling and formation of experimental allergic encephalomyelitis
Introduction: Experimental autoimmune (allergic) encephalomyelitis (EAE) induced by intradermal injection of homogenate of the brain, spinal cord and peripheral nerve with Freund’s stimulator, refers to a true autoimmune disease of the nervous system. Materials and methods: Experimental studies were conducted on white nonlinear rats. To induce experimental allergic encephalomyelitis (EAE), homologous brain homogenates was used, which leads among other drugs (homologous, heterogeneous brain and spinal cord homogenates) by encephalitogenity. The connective tissue of the animal’s tail base was injected with a mixture of encephalitogenic suspension of 0.1 ml per 100 g of the body weight. Results and discussion: According to the results, in the rats, there was weight loss, and the abnormal neurological symptoms were found on an average of 10–12th days. Our experimental studies on the formation of EAE were confirmed morphologically by electron microscopy. Conclusion: Thus, the use of this technique allowed us to obtain a simulated pathologic condition of multiple sclerosis in the form of experimental allergic encephalomyelitis and can be used in future studies to identify appropriate laws, the extent and nature of changes in the immune and nervous systems of the body when inducing experimental pathological conditions.
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来源期刊
Research Results in Pharmacology
Research Results in Pharmacology Medicine-Pharmacology (medical)
CiteScore
1.50
自引率
0.00%
发文量
32
审稿时长
12 weeks
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