SunFire C18色谱柱高效液相色谱法测定氨虫素和氨虫素的方法

IF 0.5 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

Chemija Pub Date : 2022-08-07 DOI:10.6001/chemija.v33i3.4750

Anastasiia Belikova, Anna Materienko, Ludmila Sidorenko, Olha Chorna, Deividas Burdulis, V. Georgiyants

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引用次数: 0

摘要

建立了用光电二极管阵列检测器反相高效液相色谱法测定同一样品中阿米松和阿米辛及其与抗生素(头孢曲松、四环素、氨苄西林和左氧氟沙星)混合物中阿米松和阿米辛的含量。采用SunFire C18色谱柱，流动相为高氯酸钠缓冲液(pH = 2.5)和乙腈(75:25)，流速为0.8 ml/min。分析物在205和265 nm处鉴定。方法验证时进行了特异性、线性度、精密度参数、定量限和定量限评价，结果表明:阿米唑酮和阿米辛的相关系数分别为0.9992和0.9998。本方法也可用于测定环境中抗生素混合物中阿米松和阿米辛的含量。

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Development of a method for the detection of amixin and amizon by HPLC on SunFire C18 column

A method for the determination of amizone and amixin in the same sample and in their mixture with antibiotics (ceftriaxone, tetracycline, ampicillin and levofloxacin) was developed using reversed-phase highperformance liquid chromatography equipped with a photodiode array detector. A SunFire C18 column, a mobile phase consisting of sodium perchlorate buffer (pH = 2.5) and acetonitrile (75:25), at a flow rate of 0.8 ml/min, was used. The analytes were identified at 205 and 265 nm. The specificity, linearity, precision parameters, LOD and LOQ were evaluated during the validation of the methodology, and the correlation coefficients of amizone and amixin were 0.9992 and 0.9998, respectively. This method can also be used for the determination of amizone and amixin and their presence in a mixture with antibiotics in the environment.

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来源期刊

Chemija 化学-化学综合

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

>12 weeks

期刊介绍： Chemija publishes original research articles and reviews from all branches of modern chemistry, including physical, inorganic, analytical, organic, polymer chemistry, electrochemistry, and multidisciplinary approaches.