评估发作性和慢性偏头痛动物模型中的血浆和脑TRPV1通道：体感皮层TRPV1在偏头痛转化中的可能作用

IF 0.4 Q4 NEUROSCIENCES

Archives of Neuroscience Pub Date : 2022-01-30 DOI:10.5812/ans.115709

F. Karimzadeh, Fahime Zavvari, A. Janzadeh, Negin Mojarad, Zeinab Ghorbani, M. Togha

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引用次数: 0

摘要

背景：尽管进行了广泛的研究，但偏头痛发展的确切分子机制，尤其是其从发作性到慢性的进展和转变，仍然未知。目的：本研究旨在评估体感皮层和海马瞬时受体电位香草素1（TRPV1）在偏头痛大鼠模型中的作用。方法：本研究为干预性研究。成年雄性Wistar大鼠被分为三组，包括假偏头痛、发作性偏头痛（EM）和慢性偏头痛（CM）。假手术组每两天接受一次正常腹膜内（IP）盐水注射，持续11天，EM组接受单剂量三硝基甘油（TNG）注射（IP；10mg/kg）。对于CM组，TNG每两天给药一次（第3、5、7、9和11天；IP；10mg/kg）。用酶联免疫吸附试验（ELISA）试剂盒检测血浆、体感皮层和海马中的TRPV1水平。结果：偏头痛诱导后，CM组和EM组的血浆TRPV1水平（两组均<0.001）、体感皮层（分别<0.05和P<0.001）和海马（两组都<0.01）均升高。有趣的是，在体感皮层中，CM组的TRPV1升高远大于EM组，两组之间观察到显著差异（P<0.05）。结论：我们的结果表明，头痛的严重程度和频率可能随着体感皮层TRPV1的上调而增强。这一新成果有助于开发预防CM的新药方法。

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Evaluating Plasma and Brain TRPV1 Channels in the Animal Model of Episodic and Chronic Migraine: The Possible Role of Somatosensory Cortex TRPV1 in Migraine Transformation

Background: Despite extensive research, the exact molecular mechanisms underlying migraine development and especially its progression and transformation from episodic into chronic is still unknown. Objectives: This study aimed to assess the role of somatosensory cortex and hippocampal transient receptor potential vanilloid 1 (TRPV1) in migraine in a rat model. Methods: This study was an intervention study. Adult male Wistar rats were divided into three groups, including sham, episodic migraine (EM), and chronic migraine (CM). The sham group received normal intraperitoneal (IP) saline injections every two days for 11 days, and the EM group received a single dose of trinitroglycerin (TNG) injection (IP; 10 mg/kg). For the CM group, TNG was administrated every two days (on days 3, 5, 7, 9, and 11; IP; 10 mg/kg). TRPV1 levels in plasma, somatosensory cortex, and hippocampus were detected with an enzyme-linked immunosorbent assay (ELISA) kit. Results: The findings showed that in both CM and EM groups the TRPV1 levels in plasma (P < 0.001 in both groups), somatosensory cortex (P < 0.05 and P < 0.001, respectively), and hippocampus (P < 0.01 in both groups) increased after migraine induction. Interestingly, in the somatosensory cortex, this TRPV1 elevation in the CM group was much greater than the EM group, and a significant difference was observed between the two groups (P < 0.05). Conclusions: Our results suggested that headache severity and frequency may enhance concomitant with the upregulation of somatosensory cortex TRPV1. This new achievement can help to develop new drug approaches to prevent CM.

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Archives of Neuroscience NEUROSCIENCES-

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期刊介绍： Archives of neuroscience is a clinical and basic journal which is informative to all practitioners like Neurosurgeons, Neurologists, Psychiatrists, Neuroscientists. It is the official journal of Brain and Spinal Injury Research Center. The Major theme of this journal is to follow the path of scientific collaboration, spontaneity, and goodwill for the future, by providing up-to-date knowledge for the readers. The journal aims at covering different fields, as the name implies, ranging from research in basic and clinical sciences to core topics such as patient care, education, procuring and correct utilization of resources and bringing to limelight the cherished goals of the institute in providing a standard care for the physically disabled patients. This quarterly journal offers a venue for our researchers and scientists to vent their innovative and constructive research works. The scope of the journal is as far wide as the universe as being declared by the name of the journal, but our aim is to pursue our sacred goals in providing a panacea for the intractable ailments, which leave a psychological element in the daily life of such patients. This authoritative clinical and basic journal was founded by Professor Madjid Samii in 2012.