生物体液外泌体分离的原理与问题

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2022-06-16 DOI:10.1134/S1990747822030096

E. I. Yakubovich, A. G. Polischouk, V. I. Evtushenko

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引用次数: 10

摘要

外泌体是一种小型膜细胞外囊泡，具有很大的诊断和治疗潜力，但缺乏有效分离和分析外泌体的标准化方法，限制了外泌体技术进入临床实践。本文讨论了从生物体液中分离外泌体的相关问题，以及传统和替代分离方法的原理。提出的审查的目的是说明各种方法基于物理和生化性质的外泌体，可用于外泌体的分离。讨论了不同方法的优缺点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Principles and Problems of Exosome Isolation from Biological Fluids

Exosomes, the subclass of small membrane extracellular vesicles, have great diagnostic and therapeutic potential, but the lack of standardized methods for their efficient isolation and analysis limits the introduction of exosomal technologies into clinical practice. This review discusses the problems associated with the isolation of exosomes from biological fluids, as well as the principles of traditional and alternative methods of isolation. The aim of the presented review is to illustrate the variety of approaches based on the physical and biochemical properties of exosomes that can be used for exosome isolation. The advantages and disadvantages of different methods are discussed.

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来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.