分析肝组织对局部止血剂的反应

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
V. P. Gavrilyuk, V. Lipatov, E. Mishina, D. A. Severinov
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引用次数: 1

摘要

目的——我们的研究旨在评估模拟损伤后肝组织的变化,并在体内实验过程中使用新型止血剂(含氨甲环酸)。材料和方法:研究以下止血剂:TachoComb胶原板(第1组);基于羧甲基纤维素钠(CMC)、Na-CMC的新型局部止血剂的样品(第2组);Na-CMC+氨甲环酸(第3组)。我们通过腹腔镜对30只模拟肝撕裂伤的兔子进行了研究,并通过上述方法止血。然后,在肝组织显微照片上,我们测量包膜厚度(μm)、肝细胞及其细胞核的大小(px)、双核肝细胞的数量。我们还计算了肝细胞的核质比(NCR),并对测试样品中的胶囊成分进行了定性和定量鉴定。结果-在胶原板组中,第2组和第3组样品的胶囊宽度分别是该参数的3.2倍和3.6倍。间接地,这一发现暗示了旨在限制损伤区域的更严重的炎症和再生过程,并通过测试样品周围包膜的细胞组成的统计学显著差异得到了证实(成纤维细胞在第2组和第3组中占主导地位,在第1组中较少)。在第3组中检测到最高的细胞指数(CI)值。结论-由于结缔组织生长是炎症反应的一部分,还观察到将测试材料与肝实质分隔开的显著边界区。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analyzing Liver Tissue Responses To Local Hemostatic Agents
Objective – Our study aimed at evaluating changes in liver tissues after simulated injury and use of novel hemostatic agents (containing tranexamic acid) in the course of in vivo experiment. Material and Methods – The following hemostatic agents were studies: TachoComb collagen plate (Group 1); samples of novel local hemostatic agents based on sodium carboxymethyl cellulose (CMC), Na-CMC (Group 2); Na-CMC + tranexamic acid (Group 3). We performed the study on 30 rabbits with simulated liver laceration via laparoscopy, and were stopping bleeding by means of the above-mentioned methods. Then, on liver tissue micrographs, we were measuring capsule thickness (μm), sizes of hepatocytes and their nuclei (px), number of binuclear hepatocytes. We were also calculating nuclear-cytoplasmic ratio (NCR) of hepatocytes, as well as identifying qualitative and quantitative capsule composition in tested samples. Results – In the collagen plate group, the capsule width was 3.2-fold and 3.6-fold of this parameter in samples from Groups 2 and 3. Indirectly, this finding implied more severe inflammatory and regenerative processes aimed at limiting injury area, and was confirmed by statistically significant differences in the cellular composition of the capsule surrounding tested samples (fibroblastic cells predominated in Groups 2 and 3, and there were fewer of them in Group 1). The highest cell index (CI) value was detected in Group 3. Conclusion – A significant boundary zone separating tested materials from the liver parenchyma was also observed due to connective tissue growth as part of inflammatory response.
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来源期刊
Russian Open Medical Journal
Russian Open Medical Journal MEDICINE, GENERAL & INTERNAL-
CiteScore
0.90
自引率
0.00%
发文量
39
期刊介绍: Russian Open Medical Journal (RusOMJ) (ISSN 2304-3415) is an international peer reviewed open access e-journal. The website is updated quarterly with the RusOMJ’s latest original research, clinical studies, case reports, reviews, news, and comment articles. This Journal devoted to all field of medicine. All the RusOMJ’s articles are published in full on www.romj.org with open access and no limits on word counts. Our mission is to lead the debate on health and to engage, inform, and stimulate doctors, researchers, and other health professionals in ways that will improve outcomes for patients. The RusOMJ team is based mainly in Saratov (Russia), although we also have editors elsewhere in Russian and in other countries.
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