志贺氏菌的实验室培养与贮藏

Current Protocols in Microbiology Pub Date : 2019-12-09 DOI:10.1002/cpmc.93

Shelley M. Payne

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引用次数: 3

摘要

志贺氏菌与大肠杆菌密切相关，易于在实验室中维持和储存。本文包括常规生长条件和培养基的制备、细菌的储存和毒力质粒存在的监测。©2019 by John Wiley &基本方案1:flexneri在冷冻培养基或琼脂培养基中生长基本方案2:flexneri在丰富液体培养基中生长备用方案1:flexneri在丰富液体培养基中生长备用方案2:flexneri在最小培养基中生长基本方案3:flexneri在冷冻培养基中储存备用方案3:flexneri在琼脂培养基中储存

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Laboratory Cultivation and Storage of Shigella

Shigella species, which are closely related to Escherichia coli, can easily be maintained and stored in the laboratory. This article includes protocols for preparation of routine growth conditions and media, for storage of the bacteria, and for monitoring of the presence of the virulence plasmid. © 2019 by John Wiley & Sons, Inc.

Basic Protocol 1: Growth of S. flexneri from frozen stocks or agar stabs

Basic Protocol 2: Growth of S. flexneri in rich liquid medium

Alternate Protocol 1: Growth of S. flexneri in rich defined medium

Alternate Protocol 2: Growth of S. flexneri in minimal medium

Basic Protocol 3: Storage of S. flexneri in frozen stocks

Alternate Protocol 3: Storage of S. flexneri in agar stabs

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来源期刊

Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.