近红外区反射光谱线性判别分析法在三文鱼科鱼类物种识别中的应用

V. Y. Novikov, K. Rysakova, A. Baryshnikov
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引用次数: 0

摘要

众所周知,三文鱼科鱼类的营养价值各不相同。不同种类三文鱼的解剖形态特征有一定的相似性;因此,这个家族的代表往往是伪造的。该家族鱼类产品的分类造假通常是通过用营养价值降低的廉价鱼类取代更有价值的鱼类来进行的。最常见的情况是,远东三文鱼(三文鱼、粉红三文鱼、奇努克三文鱼、升麻三文鱼)假冒大西洋三文鱼。近红外光谱(NIR)现在越来越多地用于识别和鉴定密切相关的生物体,在某些情况下是一种取代遗传分析的快速方法。我们获得了来自北部盆地的三种三文鱼科鱼类的近红外辐射的扩散反射光谱。通过分析预干燥的无脂肪肌肉组织样品的近红外光谱,获得了按鱼类种类划分的最佳分类。在湿样品的情况下,观察到的差异不太显著,相邻聚类的单个值差异不显著。利用肌肉蛋白近红外反射光谱的线性判别分析方法进行鱼类物种鉴定的可能性已经得到了证明。
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Application of the linear method of discriminant analysis of reflectance spectra in the near infrared region for the species identification of fish of the Salmonidae family
It is well known that fish belonging to the Salmonidae family differ in their nutritional value. Anatomical and morphological features of different salmon species have a certain similarity; therefore, representatives of this family are most often falsified. Assortment falsification of products from fish of this family is usually carried out by replacing more valuable species with cheaper ones with a reduced nutritional value. Most often, counterfeiting of Atlantic salmon (salmon) by Far Eastern ones (chum salmon, pink salmon, chinook salmon, coho salmon) is found. Near infrared spectroscopy (NIR) is now increasingly used for identification and authentication of closely related organisms, in some cases being a rapid method replacing genetic analysis. We have obtained diffusion reflectance spectra of NIR radiation for three species of fish from the Northern Basin belonging to the salmon family. The best classification by fish species has been obtained by analyzing the NIR spectra of pre-dried fat-free muscle tissue samples. In case of wet samples, the observed differences are less significant, up to insignificant differences in individual values from neighboring clusters. The possibility of using the method of linear discriminant analysis of the NIR reflection spectra of muscle proteins for the species identification of fish has been shown.
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