甜菊(Stevia rebaudiana)对不同浓度的噻二唑仑(TDZ)和萘乙酸(NAA)的体外反应

Muhammad Dimas, Wiwik Indrawati, Dedi Supriyatdi
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引用次数: 0

摘要

甜叶菊可以通过营养繁殖和生殖繁殖进行发育,而甜叶菊的生殖繁殖可以通过种子进行。常规提供种子或幼苗的成功率很低。通过组织培养繁殖速度更快,产生更多的种子,并且与亲本相同,因此植物的质量是可以预期的。用于组织培养的植物生长调节剂(PGR)有型生长素、细胞分裂素和赤霉素。生长素和细胞分裂素在细胞分裂、细胞伸长、细胞分化和器官形成中的作用。本研究旨在确定甜菊菊抗噻脲(TDZ)和萘乙酸(NAA)的浓度及最佳组合。研究采用随机区组设计(RBD)因子模式,包括两个因素:TDZ和NAA。第一个因素是TDZ浓度,由三个水平组成:0毫克。l-1 (T0), 1mg。l-1 (T1)和2mg。l - 1 (T2)。第二个因素是NAA浓度,它由三个水平组成:0毫克。l-1 (N0), 1mg。l-1 (N1)和2mg。l - 1 (N1)。如果使用F检验的数据分析存在差异,则继续使用5%的BNT水平检验。根据得到的研究结果,研究了TDZ生长调节剂对甜叶菊芽增殖书数的影响和NAA生长调节剂浓度对甜叶菊芽增殖书数的影响。TDZ对甜叶菊的株高、叶片和芽增殖节均有影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Respons Planlet Stevia (Stevia rebaudiana) terhadap Penambahan berbagai Konsentrasi Thidiazuron (TDZ) dan Naphthalene Acetic Acid (NAA) secara in Vitro
Stevia can be developed by vegetative and generative propagation, while the generative propagation of stevia can be done through seeds. Conventional provision of seeds or seedlings has a low success rate. Propagation via tissue culture is faster, produces more seeds, and is identical to the parent, so the plants’ quality is expected. Plant growth regulator (PGR) often used for tissue culture is type auxins, cytokinins and gibberellins. The function of auxin and cytokinin for division cells, cell elongation, cell differentiation and organ formation. This research aims to get the concentration as well as the best combination for the growth of stevia plants against the administration of thidiazuron (TDZ) and naphthalene acetic acid (NAA). Research carried out by the randomised block design (RBD) factorial pattern consisting of two factors: TDZ and NAA. The first factor is TDZ concentration consisting of three levels: 0 mg.l-1 (T0), 1 mg.l-1 (T1), and 2 mg. l-1 (T2). The second factor is NAA concentration, which consists of three levels: 0 mg.l-1 (N0), 1 mg.l-1 (N1), and 2 mg.l-1 (N1). If there is a difference in data analysis using the F test, then proceed with the BNT level test of 5%. Based on the research results obtained, the influence of TDZ growth regulators and no effect of the concentration of NAA growth regulator on stevia bud multiplication book number. There is an effect of TDZ on plantlet height, leaves, and stevia shoot multiplication nodes.
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