改良WPM培养基对桔梗分生组织增殖效果的研究。

Q3 Agricultural and Biological Sciences

Advances in horticultural science Pub Date : 2021-08-03 DOI:10.36253/ahsc-10536

Secil Kivrak Kiran, S. Galatali, Sevil Yeniocak, Damla Ekin Ozkaya, Taner Mercan, Sevinc Guldag, O. Çelįk, N. Abdul Ghafoor, Eergun Kaya

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引用次数: 2

摘要

土耳其的榛树种植主要集中在北部地区，是当地农民重要的生计来源。世界榛子产量的70%以上由土耳其供应，但与其他国家相比，土耳其的榛子生产面积相当狭窄。本研究旨在开发7个地方品种的有效离体生产技术。因此，采用Fe-EDDHA、AgNO3、H3BO3、木炭和赤霉素酸对添加6-苄基氨基嘌呤(BAP)的WPM培养基进行单独或联合改性。在WPM培养基中，添加4.4µM BAP、27.8µM Fe-EDDHA和10g/L木炭，再生率在68% ~ 94%之间。采用ISSR引物对最佳培养基分生组织培养的芽进行遗传稳定性分析，对PCR产物凝胶图像进行检测，在7个省采集的样品中均未发现多态性条带，遗传稳定性为100%。

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Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.

Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is quite narrow. In this study was aimed to develop an effective in vitro production for seven local cultivars of C. avellana. Therefore, WPM medium supplemented with 6-Benzylaminopurine (BAP) was modified by using single or in combination of Fe-EDDHA, AgNO3, H3BO3, charcoal and gibberellic acid. In all varieties, the best regeneration rates varying between 68% and 94% were obtained from WPM medium supplemented with 4.4 µM BAP, 27.8 µM Fe-EDDHA and 10g/L Charcoal. Genetic stability of shoots derived from meristem culture using the best medium was analysed using ISSR primers, when the gel images of the PCR products were examined, no polymorphic band was observed in samples collected from seven provinces, and the genetic stability was determined as 100%.

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来源期刊

Advances in horticultural science Agricultural and Biological Sciences-Horticulture

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Advances in Horticultural Science aims to provide a forum for original investigations in horticulture, viticulture and oliviculture. The journal publishes fully refereed papers which cover applied and theoretical approaches to the most recent studies of all areas of horticulture - fruit growing, vegetable growing, viticulture, floriculture, medicinal plants, ornamental gardening, garden and landscape architecture, in temperate, subtropical and tropical regions. Papers on horticultural aspects of agronomic, breeding, biotechnology, entomology, irrigation and plant stress physiology, plant nutrition, plant protection, plant pathology, and pre and post harvest physiology, are also welcomed. The journal scope is the promotion of a sustainable increase of the quantity and quality of horticultural products and the transfer of the new knowledge in the field. Papers should report original research, should be methodologically sound and of relevance to the international scientific community. AHS publishes three types of manuscripts: Full-length - short note - review papers. Papers are published in English.