益生菌联合疫苗接种对肉仔鸡抗体应答、生化和血液学指标的保护作用

IF 3.8 3区农林科学 Q1 VETERINARY SCIENCES

Pakistan Veterinary Journal Pub Date : 2019-07-01 DOI:10.29261/pakvetj/2019.023

Noreen Sarwar

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引用次数: 8

摘要

2018年12月13日2018年12月20日2018年12月26日2019年1月28日本研究研究了多菌株益生菌联合疫苗接种对抗体应答、血液化学和血液学参数的影响。将60日龄肉鸡随机分为对照组、益生菌组、疫苗组和益生菌+疫苗组。益生菌以2g/500ml水的剂量提供，持续5天/周，直至6周。第7天，以0.025ml/只滴眼液方式接种新城疫疫苗。第21天时，用0.50ml /只的蒸馏水饮用NDV Lasota疫苗。分别于试验第10、22、30天饮水接种IBDV疫苗。分别于第15、30和45天采集血样，测定抗体反应、血液化学和血液学参数。对照组和益生菌组抗新城疫疫苗和IBD疫苗的抗体滴度均下降。在接种NDV疫苗的情况下，疫苗组(GMT=log2)和益生菌+疫苗组(GMT=log2)在试验第45天无显著差异。对于IBDV疫苗，45 d时疫苗组(2233)和益生菌+疫苗组(4206)的ELISA滴度差异有统计学意义。第45天，PV组胆固醇和ALT显著降低。PV组WBC计数明显升高。P≤0.05。与疫苗一起使用益生菌可改善抗体滴度，但未观察到血液化学和血液学参数的有害增加或减少。©2018 PVJ。版权所有

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Protective Effect of Probiotics in Combination with Vaccination on Antibody Response, Biochemical and Hematological Indices in Broilers

Received: Revised: Accepted: Published online: December 13, 2018 December 20, 2018 December 26, 2018 January 28, 2019 In this study, the effect of multi strain probiotics was studied on antibody response, blood chemistry and hematological parameters in combination with vaccination. A total of sixty day-old broiler chicks were equally divided into four groups: Control, Probiotic, Vaccine and Probiotic + Vaccine. Probiotics were offered at 2g/500ml of water for 5 days/week up to 6 weeks. NDV vaccine was given by eye drops at 0.025ml/bird at day 7. At 21 day NDV Lasota vaccine was offered in drinking distil water at 0.50ml /bird. IBDV vaccine was administered by drinking water at 10, 22 and 30 day of experiment. Blood samples were collected at 15, 30 and 45 days to measure antibody response, blood chemistry and hematological parameters. Antibody titer against NDV vaccine and IBD vaccine showed decline in control and probiotic groups. In case of NDV vaccine, there was no significant difference in Vaccine group (GMT=log2 ) and Probiotic + Vaccine group (GMT=log2 ) at day 45 of experiment. For IBDV vaccine, there was significant difference at 45 day in ELISA titer of vaccine group (2233) and Probiotic+Vaccine group (4206). Cholesterol and ALT showed significant decrease in PV group at 45 day. Significant increase in WBC count was observed in PV group. Level of significance was P≤0.05. Probiotics when administered along with vaccine showed improvement in antibody titer but no harmful increase or decrease in blood chemistry and hematological parameters was observed. ©2018 PVJ. All rights reserved

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来源期刊

Pakistan Veterinary Journal 兽医-兽医学

CiteScore

4.20

自引率

13.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Pakistan Veterinary Journal (Pak Vet J), a quarterly publication, is being published regularly since 1981 by the Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan. It publishes original research manuscripts and review articles on health and diseases of animals including its various aspects like pathology, microbiology, pharmacology, parasitology and its treatment. The “Pak Vet J” (www.pvj.com.pk) is included in Science Citation Index Expended and has got 1.217 impact factor in JCR 2017. Among Veterinary Science Journals of the world (136), “Pak Vet J” has been i) ranked at 75th position and ii) placed Q2 in Quartile in Category. The journal is read, abstracted and indexed internationally.