牦牛心包在抗钙化心脏瓣膜中的应用

Jing Liu, Binhan Li, Yibo Qin, X. Leng
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摘要

目的探讨牦牛心包作为抗钙化心脏瓣膜材料在经导管主动脉瓣置换术(TAVR)中的可行性。以牛心包和市售Sino产品为对照。方法分别采用苏木精-伊红(HE)染色和DNA检测试剂盒对牦牛心包脱细胞程度进行定性和定量分析。两种交联剂,即戊二醛和吉尼平,分别用于交联脱细胞心包。对交联心包的力学性能、热力学稳定性和生物相容性进行测试后,将交联心包皮下植入幼龄Wistar大鼠。植入2~4周后进行组织学染色和组织钙化分析。结果脱细胞处理后,牦牛心包细胞核基本不可见,DNA含量由(0.90±0.13)μg/mg降至(0.09±0.02)μg/mg,显著低于Sino产品(P < 0.05)。两种交联牦牛心包的溶血率和细胞增殖率与牛心包相似(P < 0.05),皮下植入的组织再生能力较差,钙化水平高于牛心包,但差异无统计学意义(P < 0.05)。皮下植入4周后,戊二醛交联心包钙含量为32.62~65.49 μg/mg。结论牦牛心包具有较好的力学性能和热力学稳定性,其生物相容性可满足经导管主动脉瓣置换术的要求。关键词:心脏瓣膜;牦牛心包;非细胞矩阵;交联;Anti-calcification
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Application of yak pericardium in anti-calcification heart valves
Objective To explore the feasibility of the application of yak pericardium as anti-calcified heart valve materials in transcatheter aortic valve replacement (TAVR). Cattle pericardium and commercial Sino products were used as controls. Methods Yak and cattle pericardium was decellularized, and then the acellular extent was qualitatively and quantitatively analyzed with hematoxylin-eosin (HE) staining and DNA detection kit respectively. Two cross-linking agents, i.e. glutaraldehyde and genipin, were used to cross-link the acellular pericardium, respectively. After testing the mechanical property, thermodynamic stability and biocompatibility of the pericardium, the cross-linked pericardium was subcutaneously implanted in juvenile Wistar rats. After 2~4 weeks of the implantation, histological staining and tissue calcification analysis were conducted. Results The nuclei of the yak pericardium were almost invisible after decellularized treatment, and the DNA content decreased from (0.90±0.13) μg/mg to (0.09±0.02) μg/mg that was significantly lower than that of Sino product (P 0.05). For the two cross-linked yak pericardium, the hemolysis rate and cell proliferation ratio was similar with that of cattle pericardium (all P>0.05), the tissue regeneration ability of subcutaneous implantations was inferior, and the calcification level was higher than that of cattle pericardium with an no statistically significant difference (all P>0.05). After 4 weeks of the subcutaneously implantation, the calcium content of the glutaraldehyde cross-linked pericardium was 32.62~65.49 μg/mg. Conclusions Yak pericardium has better mechanical properties and thermodynamic stability than cattle pericardium, and the biocompatibility can meet the requirements of transcatheter aortic valve replacement. Key words: Heart valves; Yak pericardium; Acellular matrix; Crosslinking; Anti-calcification
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