牙龈间充质干细胞衍生的细胞外囊泡在心肌细胞急性缺氧中的作用

Q4 Medicine

Italian journal of anatomy and embryology Pub Date : 2022-12-27 DOI:10.36253/ijae-13785

Ylenia Della Rocca, J. Pizzicannella, G. Marconi, Luigia Fonticoli, O. Trubiani, F. Diomede

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引用次数: 0

摘要

缺氧对不同组织的病理状况有影响，特别是对心脏，它可以根据暴露于缺氧状态的持续时间产生不同的后果。急性缺氧暴露可导致心脏组织可逆的适应，维持良好的全身氧供应，而慢性缺氧暴露可导致组织损伤，加剧缺氧引起的心功能障碍。细胞外囊泡(EVs)是由不同细胞类型分泌的纳米级小膜囊泡。在生理和病理条件下，ev都是细胞间通讯的介质。口腔来源的间充质干细胞(MSCs)，包括人牙龈间充质干细胞，产生的EVs具有促血管生成和抗炎作用。因此，电动汽车可以确定为一个新的治疗潜力的组织再生。本研究的目的是评估人牙龈间充质干细胞(hGMSCs)产生的EVs对HL-1心肌细胞在急性缺氧状态(缺氧0.2%)和常氧条件下培养的体外模型的影响。HIF-1α的表达随着ev的作用而下调。电动汽车可能是防止缺氧损伤的创新平台。

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Role of extracellular vesicles derived by human gingival mesenchymal stem cells in cardiomyocytes acute hypoxia

Hypoxia has an impact on pathological conditions of different tissues and especially on the heart where it can have different consequences depending on the duration of exposure to the hypoxic state. Acute hypoxic exposure can result in reversible acclimatization in heart tissue, maintaining a good systemic oxygen supply, while chronic hypoxic exposure leads to tissue damage exacerbating hypoxia-induced cardiac dysfunction. Extracellular vesicles (EVs) are small membrane vesicles, of the order of nanometers, secreted by different cell types. EVs are mediators of intercellular communication in both physiological and pathological conditions. EVs produced by oral-cavity-derived Mesenchymal Stem Cells (MSCs), including human gingival mesenchymal stem cells, have pro-angiogenic and anti-inflammatory effects. For this reason, the EVs can be identify as a new therapeutic potential for tissue regeneration. The aim of the present work was to evaluate the effect of treatment with EVs produced by human gingival mesenchymal stem cells (hGMSCs) on an in vitro model of HL-1 cardiomyocytes cultured under acute hypoxia state (0,2% hypoxia) followed by normoxia conditions. The HIF-1α expression was downregulate with EVs treatment. EVs could represent an innovative platform to prevent the hypoxic damages.

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来源期刊

Italian journal of anatomy and embryology Medicine-Embryology

CiteScore

0.20

自引率

0.00%

发文量

期刊介绍： The Italian Journal of Anatomy and Embryology, founded in 1901 by Giulio Chiarugi, Anatomist at Florence University, is a peer-reviewed journal sponsored by the Italian Society of Anatomy and Embryology. The journal publishes original papers, invited review articles, historical article, commentaries, obituitary, and book reviews. Its main focus is to understand anatomy through an analysis of structure, function, development and evolution. Priority will be given to studies of that clearly articulate their relevance to the anatomical community. Focal areas include: experimental studies, contributions based on molecular and cell biology and on the application of modern imaging techniques; comparative functional morphology; developmental biology; functional human anatomy; methodological innovations in anatomical research; significant advances in anatomical education. Studies that are essentially descriptive anatomy are appropriate only if they communicate clearly a broader functional or evolutionary significance. All papers should be submitted in English and must be original works that are unpublished and not under consideration by another journal. An international Editorial Board and reviewers from the anatomical disciplines guarantee a rapid review of your paper within two to three weeks after submission.