西番莲的细胞毒性和抗肿瘤潜力分析

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2023-06-20 DOI:10.1080/10496475.2023.2225147

Chittilappilly J Anjali, C. Pareeth, T. Babu

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引用次数: 0

摘要

摘要采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)法研究了西番莲(PQ)叶片乙醇提取物对癌细胞的细胞毒性。对小鼠实体瘤和腹水瘤模型进行抗肿瘤作用分析。采用液相色谱-质谱法(LC-MS)分析了提取物的植物化学特征。荧光共振能量转移(FRET)和流式细胞术分析细胞凋亡和细胞周期。PQ对道尔顿淋巴瘤腹水(DLA)和埃利希腹水癌细胞的IC50分别为9.4和9.2 μ mL−1，对乳腺癌(MCF7)和结直肠癌(HCT116)癌细胞的IC50分别为166.5和107.09 μ mL−1具有抑制增殖作用。提取物对正常大鼠肠细胞IEC6和小鼠巨噬细胞RAW264.7均无毒性。在腹水肿瘤模型中，给药组小鼠的平均存活时间为25±4.30 d，高于未给药组(21.2±2.68 d);在dla诱导的实体模型中，给药400 mg kg−1 b.w.后，肿瘤重量由4.5±0.58 g降至1.6±0.9 g。LC-MS分析显示，染料木素、山奈酚、异牡荆素和槲皮素是已知的抗癌潜力。小鼠急性毒性实验表明，PQ是无毒的。FRET和流式细胞术分析显示pq处理的细胞诱导凋亡

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Analysis of Cytotoxic and Antitumor Potential of Passiflora quadrangularis

ABSTRACT The cytotoxicity of ethanolic extract of Passiflora quadrangularis (PQ) leaves was analyzed on cancer cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Antitumor effect was analyzed on mouse solid and ascites tumor models. The phytochemical profile of the extract was analyzed by liquid chromatography-mass spectrometry (LC-MS). Apoptosis and cell cycle were analyzed by fluorescence resonance energy transfer (FRET) and flow cytometry. PQ exhibited cytotoxicity on Dalton’s lymphoma ascites (DLA) and Ehrlich’s ascites carcinoma cells with IC50 of 9.4 and 9.2 μg mL−1 and anti-proliferative effect on breast (MCF7) and colorectal (HCT116) cancer cell lines with IC50 of 166.5 and 107.09 μg mL−1, respectively. The extract did not show toxicity in normal rat intestinal cells IEC6 and murine macrophage cells RAW264.7. In ascites tumor model, the mean survival days of the animals increased to 25 ± 4.30 d in the treated group compared to untreated (21.2 ± 2.68 d). In DLA-induced solid model, the tumor weight decreased to 1.6 ± 0.9 g from 4.5 ± 0.58 g by treatment with 400 mg kg−1 b.w. extract. LC-MS analysis revealed the presence of genistein, kaempferol, isovitexin, and quercetin that are known for anticancer potential. The acute toxicity in mice revealed that PQ is nontoxic. FRET and flow cytometry analysis indicated an induction of apoptosis in PQ-treated cells

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.