槲皮素在多囊卵巢综合征大鼠模型中调节卵巢自噬相关分子和立体参数

IF 1.7 4区医学 Q3 TROPICAL MEDICINE

Asian Pacific journal of tropical biomedicine Pub Date : 2023-01-01 DOI:10.4103/2221-1691.367686

A. Neisy, F. Koohpeyma, Majid J. Khorchani, Fatemeh Karimi, F. Zal

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引用次数: 1

摘要

目的:探讨槲皮素对多囊卵巢综合征(PCOS)大鼠卵巢体视学参数及自噬相关基因的影响。方法:50只雌性Sprague-Dawley大鼠随机分为5组:对照组、乙醇组、槲皮素组(15 mg/kg/d)、PCOS组、PCOS +槲皮素组。诱导PCOS后，口服槲皮素30 d。采用组织学、体视学和实时荧光定量PCR方法评价槲皮素对PCOS大鼠的影响。结果:体视学分析显示，与PCOS组相比，槲皮素显著增加了卵巢卵泡数量和黄体体积，导致闭锁卵泡明显减少。槲皮素显著提高mTOR基因表达，降低Beclin-1和LC3基因表达。结论:槲皮素对PCOS大鼠卵巢自噬相关基因及体视学参数的表达有明显调节作用。因此，它可以被认为是一种改善卵巢卵泡损伤的成分。

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Quercetin modulates ovarian autophagy–related molecules and stereological parameters in a rat model of PCOS

Objective: To examine the effect of quercetin on stereological parameters and autophagy-related genes in ovaries of polycystic ovary syndrome (PCOS) rats. Methods: Fifty female Sprague-Dawley rats were randomly divided into five groups: the control group, the ethanol group, the quercetin group (15 mg/kg/day), the PCOS group, as well as the PCOS + quercetin group. After the induction of PCOS, quercetin was administered orally for 30 days. Histological, stereological and real-time PCR analyses were carried out to evaluate the effect of quercetin on PCOS rats. Results: Stereological analysis revealed that quercetin significantly increased the number of ovarian follicles and the volume of corpus luteum and induced a significant decrease in atretic follicles in comparison to the PCOS group. In addition, quercetin markedly increased mTOR gene expression while decreasing Beclin-1 and LC3 gene expression. Conclusions: Quercetin strongly modulates the expression of ovarian autophagy-related genes and stereological parameters in PCOS rats. Therefore, it can be considered as an ameliorative component for ovarian follicular impairments.

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来源期刊

Asian Pacific journal of tropical biomedicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)

CiteScore

3.10

自引率

11.80%

发文量

2056

审稿时长

4 weeks

期刊介绍： The journal will cover technical and clinical studies related to health, ethical and social issues in field of biology, bacteriology, biochemistry, biotechnology, cell biology, environmental biology, microbiology, medical microbiology, pharmacology, physiology, pathology, immunology, virology, toxicology, epidemiology, vaccinology, hematology, histopathology, cytology, genetics and tropical agriculture. Articles with clinical interest and implications will be given preference.