凋亡相关基因在人卵巢颗粒细胞体外原代培养中的表达

Q4 Biochemistry, Genetics and Molecular Biology

Medical Journal of Cell Biology Pub Date : 2020-12-31 DOI:10.2478/acb-2020-0023

I. Kocherova, K. Stefańska, Rut Bryl, Joanna Perek, W. Pieńkowski, J. Žáková, I. Crha, P. Ventruba, P. Mozdziak, M. Ješeta

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引用次数: 2

摘要

摘要卵巢颗粒细胞（GC）在卵母细胞成熟过程中发挥着至关重要的作用，为卵母细胞周围创造了有利的微环境。因此，细胞凋亡增强和GC丢失可能会对卵泡内环境产生负面影响，损害卵母细胞质量，降低妊娠机会。基于RT-qPCR方法，本研究揭示了细胞凋亡相关基因（BCL2、BAX、p53、CASP9）在体外原代培养7天期间的差异表达。个体基因表达变化可能反映GC在不同时间点的存活和/或凋亡状态。运行标题：凋亡相关基因在体外颗粒细胞中的表达

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Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Abstract Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points. Running title: Apoptosis-related genes expression in granulosa cells in vitro

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来源期刊

Medical Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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