研究文章：百里香醇提物对白鲟卵的抗真菌作用与孔雀绿效果的比较

IF 0.8 4区农林科学 Q3 FISHERIES

Iranian Journal of Fisheries Sciences Pub Date : 2021-01-10 DOI:10.22092/IJFS.2021.123545

M. Shahrani, G. A. Takami, M. Rohani, A. Motallebi, Yazdani Sadati

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引用次数: 0

摘要

鲟鱼发育过程中真菌污染造成的经济损失以及孔雀石绿在环境中的副作用是非常重要的。因此，有必要研究并引入合适的天然物质来代替孔雀石绿。本研究的目的是比较寻常胸腺和孔雀石绿对从国际鲟鱼研究所获得的西伯利亚鲟鱼卵中分离的真菌的抗真菌作用。将样品在25°C的培养基（SDA）和葡萄糖肽琼脂中接种5-7天，然后鉴定分离的真菌。采用纸片扩散法评价了寻常T.vulgaris提取物的抗真菌作用，并测定了真菌的MIC和MFC。在本研究中。，镰刀菌属。，红酵母属。，青霉属。，木霉属。，从金孢霉受精卵中分离鉴定了金孢菌属和无菌菌丝。结果表明，T.vulgaris提取物对所有分离的真菌都有显著的影响。最有效的样品是Saprolegnia，最不有效的是镰刀菌和木霉。Saprolegnia spp。，镰刀菌属、红酵母属、。，青霉属。，木霉属。，金孢菌属。，无菌菌丝分别为≥0.75、3、1.5、1.5、0.75、1.5、≤0.75 mg/ml和≥1.5、≥3、3、1、5、3和1.5 mg/ml。由于Saprolegnia spp。在A.baerii卵中，以及T.vulgaris酒精提取物的显著作用，它可以作为孔雀石绿的良好替代品，以减少A.baeriii繁殖季节真菌污染的频率及其经济损失。

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Research Article: Antifungal effects of alcoholic extract of Thymus vulgaris on Siberian sturgeon (Acipenser baerii) eggs compared with malachite green effects

The economic losses due to fungal contamination in development of sturgeon and the side effects of malachite green in the environment are very important. Therefore, it is essential to investigate and introduce suitable natural substances instead of malachite green. The aim of this study was to compare antifungal effect of Thymus vulgaris with malachite green on fungi isolated from Siberian sturgeon eggs obtained from International Sturgeon Research Institute. The samples were inoculated in culture media (SDA) and Glucose-Peptone Agar at 25°C for 5-7 days and then isolated fungi were identified. The antifungal effect of T. vulgaris extract was evaluated by disc diffusion method and MIC and MFC of fungi was measured. In this study, Saprolegnia spp ., Fusarium spp ., Rhodotorula spp ., Penicillium spp ., Trichoderma spp ., Chrysosporium spp .and sterile hyphaewere isolated and identified from fertilized eggs of A. baerii. According to the results, T. vulgaris extract had a significant effect on all isolated fungal species. The most effective sample was observed on Saprolegnia and the least effective ones were Fusarium and Trichoderma. The MIC and MFC of alcoholic extract for Saprolegnia spp ., Fusarium spp., Rhodotorula spp ., Penicillium spp ., Trichoderma spp ., Chrysosporium spp ., sterile hyphae were ≥0.75, 3, 1.5, 1.5, 0.75, 1.5, ≤0.75 mg/ml, and ≥1.5, ≥3, 3, 3, 1.5, 3 and 1.5 mg/ml, respectively. Due to high frequency of Saprolegnia spp . in A. baerii eggs, and the significant effect of alcoholic extract of T. vulgaris, it could be used as a good alternative for malachite green to reduce the frequency of fungal contamination and its economic losses during reproductive seasons of A. baerii.

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来源期刊

Iranian Journal of Fisheries Sciences FISHERIES-

CiteScore

2.30

自引率

11.10%

发文量

审稿时长

3 months

期刊介绍： 1- Living various species (contains animals and vegetal species) in various aquatic ecosystems. 2- Health and diseases of aquatic species. 3- Determining the stocks and specific time and location for catching and reliable exploitation for sustainable development. 4- Methods of propagation and culture of high value aquatic resources. 5- Aquatic stock assessment and the methods of restocking the high value species and suggestion for rate, areas and the time for releasing fish and other aquatic organisms fries. 6- Pollutant agents and their effects to the environments of aquatic species. 7- Feed and feeding in aquatic organisms. 8- Fish processing and producing new products. 9- The economic and social aspects of fisheries.