具有复制能力的MGF110（1L-5-6L）缺失非洲猪瘟病毒（基因型II）的设计

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria-Beograd Pub Date : 2023-03-01 DOI:10.2478/acve-2023-0002

M. Nefedeva, A. Malogolovkin, I. Titov

{"title":"具有复制能力的MGF110（1L-5-6L）缺失非洲猪瘟病毒（基因型II）的设计","authors":"M. Nefedeva, A. Malogolovkin, I. Titov","doi":"10.2478/acve-2023-0002","DOIUrl":null,"url":null,"abstract":"Abstract Viral individual genes functions and their role in the interaction with the host cells remain the main area in the study of African swine fever virus (ASFV) biology. The extreme heterogeneity of the ASFV makes it difficult to develop vaccines against this pathogen. In this work, we generated the ASFV deletion mutant virus Volgograd/D(1L-5-6L) with the six genes deletion in multigenic family 110 (MGF110) (1L-5-6L) and studied its characteristics in vitro. The homologous recombination method was used for the deletion in ASFV parental strain Volgograd/14с. A series of six passages was carried out in the COS-1 cell culture using the limiting dilution method. The recombinant strain Volgograd/D(1L-5-6L) MGF110 was selected by the plaque formation method. Performed study of viral replication showed no changes in viral growth kinetics in comparison with the parental strain. The ASFV Volgograd/D(1L-5-6L) MGF110 is a great tool available to test the importance of MGF110 for virus virulence and vaccine development.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II)\",\"authors\":\"M. Nefedeva, A. Malogolovkin, I. Titov\",\"doi\":\"10.2478/acve-2023-0002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Viral individual genes functions and their role in the interaction with the host cells remain the main area in the study of African swine fever virus (ASFV) biology. The extreme heterogeneity of the ASFV makes it difficult to develop vaccines against this pathogen. In this work, we generated the ASFV deletion mutant virus Volgograd/D(1L-5-6L) with the six genes deletion in multigenic family 110 (MGF110) (1L-5-6L) and studied its characteristics in vitro. The homologous recombination method was used for the deletion in ASFV parental strain Volgograd/14с. A series of six passages was carried out in the COS-1 cell culture using the limiting dilution method. The recombinant strain Volgograd/D(1L-5-6L) MGF110 was selected by the plaque formation method. Performed study of viral replication showed no changes in viral growth kinetics in comparison with the parental strain. The ASFV Volgograd/D(1L-5-6L) MGF110 is a great tool available to test the importance of MGF110 for virus virulence and vaccine development.\",\"PeriodicalId\":55559,\"journal\":{\"name\":\"Acta Veterinaria-Beograd\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2023-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Veterinaria-Beograd\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2478/acve-2023-0002\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Veterinaria-Beograd","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2478/acve-2023-0002","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

病毒个体基因的功能及其在与宿主细胞相互作用中的作用仍然是非洲猪瘟病毒生物学研究的主要领域。非洲猪瘟病毒的极端异质性使得开发针对这种病原体的疫苗变得困难。本研究构建了多基因110家族(MGF110) (1L-5-6L)中6个基因缺失的ASFV缺失突变病毒Volgograd/D(1L-5-6L)，并对其体外特性进行了研究。采用同源重组法对ASFV亲本株Volgograd/ 14vir进行基因缺失。用极限稀释法对COS-1细胞进行连续6代培养。通过斑块形成法选择重组菌株Volgograd/D(1L-5-6L) MGF110。进行的病毒复制研究表明，与亲本菌株相比，病毒生长动力学没有变化。ASFV Volgograd/D(1L-5-6L) MGF110是测试MGF110对病毒毒力和疫苗开发重要性的重要工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II)

Abstract Viral individual genes functions and their role in the interaction with the host cells remain the main area in the study of African swine fever virus (ASFV) biology. The extreme heterogeneity of the ASFV makes it difficult to develop vaccines against this pathogen. In this work, we generated the ASFV deletion mutant virus Volgograd/D(1L-5-6L) with the six genes deletion in multigenic family 110 (MGF110) (1L-5-6L) and studied its characteristics in vitro. The homologous recombination method was used for the deletion in ASFV parental strain Volgograd/14с. A series of six passages was carried out in the COS-1 cell culture using the limiting dilution method. The recombinant strain Volgograd/D(1L-5-6L) MGF110 was selected by the plaque formation method. Performed study of viral replication showed no changes in viral growth kinetics in comparison with the parental strain. The ASFV Volgograd/D(1L-5-6L) MGF110 is a great tool available to test the importance of MGF110 for virus virulence and vaccine development.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Acta Veterinaria-Beograd 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

18-36 weeks

期刊介绍： The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.