锥束计算机断层扫描(CBCT)检测原发性牙髓感染中细菌类型/细菌数量与骨质流失的相关性

IF 0.1 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Ricardo Oliva Rodríguez, Georgina Yaretzy Rangel Galván, Ana María González Amaro, Francisco Javier Gutiérrez Cantú, Abraham Israel Muñoz Ruiz, José Obed García Corté, Jairo Mariel Cárdena
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引用次数: 0

摘要

根管起源的根尖周病变(PL)是影响根周组织的最常见的病理状况之一。本研究的主要目的是评估从坏死牙髓中分离的微生物数量和种类,建立这些微生物与根尖周病变大小之间的相关性,以及非手术根管治疗如何减少微生物数量和物种。选择了27名临床诊断为牙髓坏死和慢性根尖周病变的患者;锥形束计算机断层扫描(CBCT)和根管系统的微生物样本在消毒方案、器械后/消毒方案和药物后放置之前采集。通过API-20 Strep/API-20A系统对样品进行菌落形成单位(CFU)计数、革兰氏染色技术和细菌鉴定。API系统在仪器前样品中鉴定了21种微生物,在仪器后样品中鉴定出11种,在药物后样品中识别出11种。病变的初始大小与细菌数量之间的相关系数为0.598%,确定系数高达35.7%,根尖周病变的大小与CFU数量之间的相关性系数为0.486%,确定系数为23.6%。这项研究有助于了解从坏死牙髓中分离和鉴定的微生物的数量和种类,建立微生物数量和种类与根尖周病变大小之间的相关性,并表明微生物的减少如何有助于PL的愈合,证实了充分消毒方案的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Correlation between bacterial type/bacterial quantity and bone loss detected by cone beam computed tomography (CBCT) in primary endodontic infections.
Periapical lesions (PL) of endodontic origin are one of the most common pathological conditions that affect peri-radicular tissues. The main ob-jective of this study was to evaluate the amount and species of microorganisms isolated from necrotic pulps, establish a correlation between these and the size of periapical lesions, and how the amount and species of microorganisms decreased with non-surgical root canal treatment. Twenty-seven patients with a clinical diag-nosis of dental pulp necrosis and chronic periapical lesions were selected; a Cone Beam Computed Tomography (CBCT) and microbial samples of the root canal system were taken previous to a disinfection protocol, a post-instrumentation/disinfection protocol, and a post-medication placement. Samples were processed for colony-forming unit (CFU) counting, Gram staining technique, and bacterial identification by the API-20 Strep/API-20A system. The API system identified 21 species of microorganisms in the pre-instrumentation samples, 11 species in the post-instrumentation samples, and 11 in the post-medication samples. There was a correlation coefficient of 0.598% between the initial size of the lesion and the number of bacteria, with a coefficient of determination up to 35.7%, a correla-tion coefficient of 0.486% and a determination coefficient of 23.6% between the size of the periapical lesion and the number of CFUs. This study contributes to the knowledge of the amount and species of microorganisms isolated and identi-fied from necrotic pulps, establishes a correlation between the amount and spe-cies of microorganisms and the size of the periapical lesions, and shows how the decrease of microorganisms contributes to the healing of PL, corroborating the importance of an adequate disinfection protocol.
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来源期刊
Investigacion clinica
Investigacion clinica MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
0.20
自引率
50.00%
发文量
2
审稿时长
>12 weeks
期刊介绍: Estudios humanos, animales y de laboratorio relacionados con la investigación clínica y asuntos conexos.
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