尼日利亚地方鸡免疫能力溶细胞和潜伏免疫反应阶段B座β 2 (BLB2)基因的表达

A. Bello-ibiyemi, A. Adenaike, M. Takeet, A. Ubong, C. Ikeobi
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引用次数: 0

摘要

家禽的一个经济上重要的特征是免疫反应,基因鉴定仍然是一个挑战。本研究的目的是定量尼日利亚土鸡在细胞溶解和潜在免疫反应阶段的主要组织相容性复合体(MHC)II类BLB2基因的表达。共有108只尼日利亚土鸡(NIC)来自尼日利亚西南部各州。用绵羊红细胞(SRBC)接种这些鸟,然后获得血液样本(接种后5天),并进行抗体-血凝试验,将这些鸟分为抗体滴度高和抗体滴度低的组。对这些鸟类的分类产生了六组正常羽毛高、正常羽毛低、裸颈高、裸颈低、卷曲羽毛高和卷曲羽毛低抗体组。共从后代中选择48只小鸡进行基因表达研究。对胸腺和脾脏进行了外科切除,以检测鸟类的细胞溶解和潜在反应。β-肌动蛋白作为内源性对照,并采用临界阈值法(2–ΔC)测定倍数变化。与潜伏反应294.07相比,鸟类在细胞裂解免疫反应时脾脏组织表达的倍数变化为30362.44;在鸟类的细胞溶解性免疫反应中胸腺表达的倍数变化为51.98,而潜伏反应为5.24。在对SRBC抗原免疫反应的细胞溶解和潜伏阶段,脾脏中BLB2的表达相对高于胸腺,转录活性的高度与细胞溶解阶段有关。高滴度的鸟类在细胞溶解反应和潜伏反应中都有较高的mRNA表达。本研究得出结论,BLB2基因在尼日利亚土鸡中的表达在细胞溶解期被诱导,在潜伏期被抑制。在鸟类感染期间,高免疫反应鸟类的表现会比低免疫反应鸟类更好;以及BLB2基因从一个时间点到另一个时间点将被抑制的保护反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression of B Locus Beta 2 (BLB2) Gene at Cytolytic and Latent Immune Response Stages of Immunocompetence in Nigerian Indigenous Chickens
An economically-important trait in poultry for which gene identification continues to be a challenge is immune response. The objective of the study is to quantitate the expression of major histocompatibility complex (MHC) class II BLB2 gene at cytolytic and latent immune response stages in Nigerian indigenous chickens. A total of 108 Nigerian indigenous chickens (NIC) were sourced across the South-western states in Nigeria. The birds were inoculated with sheep red blood cells (SRBC), after which blood samples were obtained (5 days post-inoculation) and antibody haemagglutination test was carried out to place the birds into groups of high and low antibody titre levels. The categorisation of the birds resulted in six groups of normal feather high, normal feather low, naked neck high, naked neck low, frizzle feather high and frizzle feather low antibody groups. A total of 48 chicks were selected from the progeny for gene expression studies. Surgical excision of thymus and spleen was carried out for the detection of cytolytic and latent responses of the birds. β-actin was used as the endogenous control and the critical threshold method (2–ΔΔCт) was carried out for the determination of fold change. The fold change of spleen tissue expression at cytolytic immune response of the birds was 30,362.44 compared to latent response 294.07; and the fold change of thymus expression at cytolytic immune response of the birds was 51.98 compared to latent response 5.24. At both cytolytic and latent stages of immune response to SRBC antigen, BLB2 expression in the spleen was comparatively higher than in the thymus and the height of transcriptional activity was associated with the cytolytic stage. The birds of high titre at both the cytolytic and the latent responses had higher mRNA expression. This study concluded that BLB2 gene expression in the Nigerian indigenous chicken was induced at the cytolytic stage and repressed at the latent stage. During avian infections, the category of high immune response birds would perform better than the low immune response counterpart; and the protective response that BLB2 gene will be repressed from one time point to the other.
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