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Current Protocols in Cytometry Pub Date : 2019-06-20 DOI:10.1002/cpcy.48

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摘要

Cover:在Zhao等人(https://doi.org/10.1002/cpcy.55)中，检测由拓扑替康(Tpt)或依托泊苷(Etp)诱导Ser-139磷酸化的组蛋白H2AX (γH2AX)与细胞周期中细胞位置的关系。如前所述，未处理(Ctrl)或0.15 μM Tpt或10 μM Etp培养2小时的TK6细胞显示了双变量细胞DNA含量与γH2AX免疫荧光(IF)分布(散点图)。(Tanaka et al.， 2007)看到e55。

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Cover: In Zhao et al. (https://doi.org/10.1002/cpcy.55), Detection of histone H2AX phosphorylated on Ser-139 (γH2AX) induced by topotecan (Tpt) or etoposide (Etp) in relation to cell position in the cell cycle. Bivariate cellular DNA content versus γH2AX Immunofluorescence (IF) distributions (scatterplots) are shown for TK6 cells left untreated (Ctrl) or treated in culture with 0.15 μM Tpt or 10 μM Etp for 2 hr, as previously described (Tanaka et al., 2007). See e55.

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来源期刊

Current Protocols in Cytometry Health Professions-Medical Laboratory Technology

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期刊介绍： Published in affiliation with the International Society for Advancement of Cytometry, Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available.