RAPD标记揭示了刺菊苣与蒲公英的遗传变异。；希腊的一种重要药用植物

4区医学 Q4 Agricultural and Biological Sciences

Progress in Nutrition Pub Date : 2018-08-06 DOI:10.23751/PN.V20I1-S.5993

M. Shidfar, S. Keskin, E. Khah, Petropoulos Spiridon, F. A. Özdemir, Ibrahim Samet Gokcen

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引用次数: 3

摘要

利用15个随机扩增多态DNA（RAPD）标记对地中海盆地（希腊）5个特有属的遗传多样性和亲缘关系进行了测定。采集刺菊苣属植物3种；两个来自克里特岛（希腊），一个来自凯思诺斯岛，另外两个物种为蒲公英属（菊科）；来自奥霍姆诺斯和雅典。对240个扩增产物和163个RAPD条带进行了评分，平均67.91%的条带揭示了材料间的多态性。在本研究中，具有24条带的OPD-05引物显示出最高的带数，而具有8条带的OPM-18和OPB-16引物显示出最少的带数。具有18条多态性条带的OPV-06引物也显示出最高的条带数。在OPX-18中发现的多态带数量最少。UBC-292、OPAN-01、OPB-16、OPM-18、OPD-05引物。随后，具有29.16%的OPD-05引物显示出最低的多态性程度，而具有87.50%的OPM-18和OPB-16引物显示出最高的多态性百分比。基于多态性RAPD带数据的UPGMA聚类显示，两个不同的类群在32%的相似性水平上结合形成一个主要聚类。刺菊苣、克里特岛刺菊苣和Kythnos刺菊苣，具有100%相似性的品种也是同义词。RAPD树状图的相似性指数平均在30%至100%之间，足以表明遗传多样性和植物育种的有用变异性。

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RAPD markers reveal genetic variation between Cichorium spinosum L. and Taraxacum sp.; a substantial medicinal plants of Greece

Fifteen Random Amplified Polymorphic DNA (RAPD) markers were used to measure genetic diversity and genetic relationships between five endemic genera of Mediterranean basin (Greece). Three species of Cichorium spinosum were collected; two from island Crete (Greece) and one from island Kythnos, and other two species of Taraxacom sp. (Asteraceae); are from Orhomenos and Athens. Two hundred-forty amplified products and 163 RAPD bands were scored with an average of 67.91% of them revealing polymorphism across accessions. In this research OPD-05 primer with 24 bands showed the highest number of bands, while the OPM-18 and OPB-16, both of them with 8 bands showed the least number of bands. Also OPV-06 primer with 18 polimorphic bands showed the highest number of bands. The least number of polymorphic bandswere found in OPX-18. UBC-292, OPAN-01, OPB-16, OPM-18, OPD-05 primers. Subsequently, OPD-05 primer with 29.16% showed the least percentage of polymorphism degree, whereas OPM-18 and OPB-16 primers with 87.50% showed the highest percentage of polymorphism. UPGMA clustering based on data from polymorphic RAPD bands revealed two distinct group which joined to form one major cluster at 32% level of similarity. Also Cichorium spinosum, Crete and Cichorium spinosum, Kythnos, varieties with 100% similarity are synonyms. The similarity indices of the RAPD dendrogram ranged between 30% and 100% averagely high enough to suggest useful variability for genetic diversity and plant breeding.

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来源期刊

Progress in Nutrition 医学-营养学

CiteScore

1.40

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Progress in Nutrition was founded in 1999 as an independent magazine, a multidisciplinary approach, dedicated to issues of nutrition and metabolism.