秋明地区吸血双翅目动物病毒性疾病病原遗传物质的检测

K. Krutko, A. Kinareikina, M. I. Serkova, E. Silivanova, O. A. Fedorova
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引用次数: 1

摘要

这项研究的目的是测试在秋明地区收集的吸血蚊子是否有导致活体动物危险疾病的病毒遗传物质。材料和方法。2021年5月至10月,在秋明地区十个区的牧场和农场采集了由表格键确定物种成员的直翅目吸血昆虫。在根据分类归属、采集时间和地点从捕获的昆虫形成的60个样本中,通过聚合酶链式反应(PCR)实时评估了牛白血病原病毒、结节性皮炎病毒和非洲猪瘟病毒的遗传物质的存在。结果和讨论。采集用于PCR分析的成年昆虫为吸血蝇(蝇科、口蝇属)、蚊子(库蚊科、伊蚊属)、吸浆虫(Simuliidae科、Byssodon属和Schoenbaueria属)、马蝇(Tabanidae科,Hybomitra属、Tabanus属和Haematota属)和咬人的吸浆虫。作为对样品的牛白血病前病毒DNA的PCR检测的结果,13个样品中有1个(7.7%)的口霉属(Stomoxys spp。在吸血昆虫中检测到的牛白血病前病毒DNA表明,在昆虫采集区存在这种病原体,以及它们可能参与了其传播。考虑到秋明地区的自然和气候特征,还需要进一步研究气孔菌和Hybomitra spp.的体内媒介能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region
The purpose of the research is to test blood-sucking dipterans collected in the Tyumen Region for genetic material of viruses that cause dangerous diseases in live-stock animals.Materials and methods. From May to October 2021, blood-sucking insects of the Diptera order whose species membership was established by tabular keys were collected on pastures and farms in ten Tyumen Region districts. In 60 samples formed from the captured insects according to the taxonomic affiliation and the period and place of collection, the presence of genetic material of the leukemia provirus and the dermatitis nodularis virus of cattle (bovine) and the African swine fever virus was evaluated by polymerase chain reaction (PCR) in real time.Results and discussion. Adult insects collected for the PCR analysis were blood-sucking flies (family Muscidae, genus Stomoxys), mosquitoes (family Culicidae, genus Aedes), midges (family Simuliidae, genera Byssodon and Schoenbaueria), horseflies (family Tabanidae, genera Hybomitra, Tabanus and Haematopota), and biting midges (family Ceratopogonidae, genus Culicoides). As a result of the PCR testing of the samples for the bovine leukemia provirus DNA, 1 out of 13 samples of Stomoxys spp. (7.7%) and 1 of 13 samples of Hybomitra spp. (7.7%) were positive. The bovine leukemia provirus DNA detected in blood-sucking insects indicates the presence of this pathogen in the insect collection area as well as their possible involvement in its spread. Further research is needed on the Stomoxys spp. and Hybomitra spp. vector competence in vivo, considering natural and climatic features of the Tyumen Region.
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