一种新型肽Athycaltide-1治疗异丙肾上腺素诱导的病理性心肌肥大的靶点分析

IF 3.4 4区医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiovascular Therapeutics Pub Date : 2022-05-02 DOI:10.1155/2022/2715084

Xi Zheng, Fuxiang Su, Ze-Chun Kang, Jingyuan Li, Chenyang Zhang, Yujia Zhang, Liying Hao

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引用次数: 2

摘要

心肌肥大是许多心脏病的病理特征。这是一个复杂的过程，涉及心脏中所有类型的细胞以及与循环细胞的相互作用。本研究旨在鉴定异丙肾上腺素（ISO）诱导的心肌肥大大鼠心肌组织中的差异表达蛋白（DEPs），并探讨其改善机制。ITRAQ比较了对照组、ISO组和ATH-1组三种不同的心脏状态。成对比较显示，ISO/对照组中有121个DEP（96个上调，25个下调），ATH-1/ISO中有47个DEP，27个上调，20个下调）和ATH-1/对照组有116个DEP。然后使用STRING软件进行蛋白质网络分析。功能分析显示，Hspa1蛋白、氧化应激和MAPK信号通路在心肌肥大的发生和发展中起着重要作用，这一点在体内模型中得到了进一步验证。研究表明，ATH-1可以降低肥厚心肌中Hspa1蛋白的表达和氧化应激水平，并进一步抑制p38 MAPK、JNK和ERK1/2的磷酸化。

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Analysis of Therapeutic Targets of A Novel Peptide Athycaltide-1 in the Treatment of Isoproterenol-Induced Pathological Myocardial Hypertrophy

Myocardial hypertrophy is a pathological feature of many heart diseases. This is a complex process involving all types of cells in the heart and interactions with circulating cells. This study is aimed at identifying the differentially expressed proteins (DEPs) in myocardial hypertrophy rats induced by isoprenaline (ISO) and treated with novel peptide Athycaltide-1 (ATH-1) and exploring the mechanism of its improvement. ITRAQ was performed to compare the three different heart states in control group, ISO group, and ATH-1 group. Pairwise comparison showed that there were 121 DEPs in ISO/control (96 upregulated and 25 downregulated), 47 DEPs in ATH-1/ISO (27 upregulated and 20 downregulated), and 116 DEPs in ATH-1/control (77 upregulated and 39 downregulated). Protein network analysis was then performed using the STRING software. Functional analysis revealed that Hspa1 protein, oxidative stress, and MAPK signaling pathway were significantly involved in the occurrence and development of myocardial hypertrophy, which was further validated by vivo model. It is proved that ATH-1 can reduce the expression of Hspa1 protein and the level of oxidative stress in hypertrophic myocardium and further inhibit the phosphorylation of p38 MAPK, JNK, and ERK1/2.

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来源期刊

Cardiovascular Therapeutics 医学-心血管系统

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Cardiovascular Therapeutics (formerly Cardiovascular Drug Reviews) is a peer-reviewed, Open Access journal that publishes original research and review articles focusing on cardiovascular and clinical pharmacology, as well as clinical trials of new cardiovascular therapies. Articles on translational research, pharmacogenomics and personalized medicine, device, gene and cell therapies, and pharmacoepidemiology are also encouraged. Subject areas include (but are by no means limited to): Acute coronary syndrome Arrhythmias Atherosclerosis Basic cardiac electrophysiology Cardiac catheterization Cardiac remodeling Coagulation and thrombosis Diabetic cardiovascular disease Heart failure (systolic HF, HFrEF, diastolic HF, HFpEF) Hyperlipidemia Hypertension Ischemic heart disease Vascular biology Ventricular assist devices Molecular cardio-biology Myocardial regeneration Lipoprotein metabolism Radial artery access Percutaneous coronary intervention Transcatheter aortic and mitral valve replacement.