双相培养体系和活性炭对苹果离体繁殖的影响轧机。

Q3 Agricultural and Biological Sciences

Advances in horticultural science Pub Date : 2021-11-11 DOI:10.36253/ahsc-11825

V. Sota, C. Benelli, Brunilda Çuko, Efigkeni Kongjika

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引用次数: 2

摘要

比较了双相(固/液)培养体系(DPS)与常规(固)培养体系(CS)的效果，以及两种体系中不同浓度活性炭对苹果(Malus sylvestris)微增殖的促进作用。轧机。被调查。本研究以侧枝为初代外植体，比较了DPS和CS两种微繁体系的再生和生根能力。同时，研究了生根阶段活性炭浓度(0、250、500、1000 mg l—1)对两种微繁体系的影响。所有评估的生物特征参数在DPS繁殖体系中均较高。在两种体系中，添加活性炭都能有效地诱导根的发生，而在添加500 mg l—1活性炭的DPS体系中，根长最高(13.16 cm)。DPS培养系统是一种低成本、省时的培养技术，可提高野生苹果大批量同质繁殖的效率。

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Effect of a double phase culture system and activated charcoal on in vitro propagation of Malus sylvestris (L.) Mill.

The effectiveness of a double phase (solid/liquid) culture system (DPS) in comparison to a conventional (solid) system (CS) as well as, the role of various concentrations of activated charcoal in both systems on the enhancement of micropropagation of Malus sylvestris (L.) Mill. were investigated. In this study, lateral shoots were used as primary explants and a comparison for shoots regeneration and rooting abilities was assessed between DPS and CS micropropagation systems. Also, the effect of activated charcoal concentration (0, 250, 500, 1000 mg l-1) during rooting stage was evaluated for both micropropagation systems. All assessed biometric parameters were higher in the DPS propagation system. The addition of activated charcoal induced effectively rhizogenesis in both systems, whereas the highest value of roots length (13.16 cm) was in the DPS system supplemented with activated charcoal at 500 mg l-1. The DPS culture system represents a promising low-cost and time-saving technique which may improve micropropagation efficiency in producing a large quantity of homogenous wild apple plants.

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来源期刊

Advances in horticultural science Agricultural and Biological Sciences-Horticulture

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Advances in Horticultural Science aims to provide a forum for original investigations in horticulture, viticulture and oliviculture. The journal publishes fully refereed papers which cover applied and theoretical approaches to the most recent studies of all areas of horticulture - fruit growing, vegetable growing, viticulture, floriculture, medicinal plants, ornamental gardening, garden and landscape architecture, in temperate, subtropical and tropical regions. Papers on horticultural aspects of agronomic, breeding, biotechnology, entomology, irrigation and plant stress physiology, plant nutrition, plant protection, plant pathology, and pre and post harvest physiology, are also welcomed. The journal scope is the promotion of a sustainable increase of the quantity and quality of horticultural products and the transfer of the new knowledge in the field. Papers should report original research, should be methodologically sound and of relevance to the international scientific community. AHS publishes three types of manuscripts: Full-length - short note - review papers. Papers are published in English.