苦瓜性连锁分子标记的鉴定

IF 1 4区生物学 Q3 PLANT SCIENCES

Indian Journal of Genetics and Plant Breeding Pub Date : 2022-02-25 DOI:10.31742/ijgpb.82.1.17

Gajala . Ameen, J. Tiwari, V. Prakash, Vivek Kumar Sandilya, B. Das

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引用次数: 0

摘要

雌雄异株棘葫芦(Momordica dioica Roxb.)的主要缺点是在早期发育阶段区分植株是雄性还是雌性。调查旨在通过鉴定和验证性别连锁分子标记来消除上述限制。利用12条特异的SSR引物，筛选出雌雄各26个基因型。只有两个SSR引物MdSSR 5(刺葫芦MADS-box基因)和CMbSSR 6(黄瓜MADS-box基因)只在雌性基因型中扩增和多态性。这些特异的雌性连锁标记表明存在影响棘葫芦性别发育的非表观遗传因素。

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Identification of sex linked molecular markers in spine gourd (Momordica dioica Roxb.)

The major drawback of a dioecious spine gourd (Momordica dioica Roxb.) is distinguishing whether the plant is male or female at an earlier development stage. An investigation was aimed to eliminate aforesaid limitation by identifying and validating sex linked molecular marker. Twenty six male and female genotypes of each sex were screened with 12 specific SSR primers. Only two SSR primers, MdSSR 5 (MADS-box gene of spine gourd) and CMbSSR 6 (MADS-box gene of cucumber) showed amplification and polymorphism in female genotypes only. These specific female linked markers manifest existent of non-epigenetic factors entailed in gender development in the spine gourd.

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.