麝香Čelak和毛蕊草水醇提取物抗氧化和抗癌活性的评价

IF 3.4 Q2 TOXICOLOGY

Journal of Toxicology Pub Date : 2022-03-30 DOI:10.1155/2022/1924265

Zahra Barmoudeh, M. T. Ardakani, A. Doustimotlagh, H. Bardania

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引用次数: 8

摘要

采用不同的方法研究了胸腺草(Thymus daenensis Celak, TDC)和毛毛竹(Stachys pilifera Benth, SPB)植物水醇提取物对HepG2细胞株的影响。采用MTT法、AnnV/PI细胞凋亡法检测提取物的细胞毒性和细胞凋亡，采用总硫醇和谷胱甘肽过氧化物酶(GPX)法检测提取物的抗氧化能力。结果显示，与TDC提取物相比，SBP提取物具有更强的细胞毒性，并且随着时间的推移而增加(24和72 h的IC50值分别为128.49µg/mL和107.11µg/mL)。虽然AnnV/PI细胞凋亡实验显示两种提取物均诱导凋亡，但caspase-3活性实验显示，与对照和SPB提取物相比，TDC提取物显著提高了caspase-3活性。SPB提取物对GPX活性的提高表明其具有较高的抗氧化能力。综上所述，TDC和SPB具有较高的抗氧化能力，对HepG2癌细胞具有较高的细胞毒性，具有较高的药用价值。

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Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Thymus daenensis Čelak and Stachys pilifera Benth

Herein, the effects of hydroalcoholic extracts of Thymus daenensis Celak (TDC) and Stachys pilifera Benth (SPB) plants on HepG2 cell line were investigated by using different analyses. Cytotoxicity and apoptosis of extracts were investigated by MTT method, AnnV/PI apoptosis assay, and their antioxidant capacity was evaluated by total thiol and glutathione peroxidase (GPX) assay. The results revealed that the SBP extract was more cytotoxic compared with the TDC extract and increased over time (128.49 µg/mL vs 107.11 µg/mL IC50 values for 24 and 72 h, respectively). Although, AnnV/PI apoptosis assay showed apoptosis induction for both extracts, but the caspase-3 activity assay revealed that TDC extract significantly increased caspase-3 activity compared with the control and SPB extract. Increasing the activity of GPX by SPB extract revealed that it has high antioxidant capacity. In conclusion, the TDC and SPB with high antioxidant capacity have high cytotoxicity against HepG2 cancer cells and have high capability as a medicinal plant.

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来源期刊

Journal of Toxicology TOXICOLOGY-

CiteScore

5.50

自引率

3.40%

发文量

审稿时长

10 weeks

期刊介绍： Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.