利用磁力和喷射器进行基因转移的基础研究

IF 0.2 Q4 PHYSICS, APPLIED

Progress in Superconductivity and Cryogenics Pub Date : 2017-03-31 DOI:10.9714/PSAC.2017.19.1.009

T. Hasegawa, H. Nakagami, Y. Akiyama, S. Nishijima

{"title":"利用磁力和喷射器进行基因转移的基础研究","authors":"T. Hasegawa, H. Nakagami, Y. Akiyama, S. Nishijima","doi":"10.9714/PSAC.2017.19.1.009","DOIUrl":null,"url":null,"abstract":"Recently, DNA vaccination is attracting attentions as a new therapeutic method for lifestyle diseases and autoimmune diseases. However, its clinical applications are limited because a safe and efficient gene transfer method has not been established yet. In this study, a new method of gene transfer was proposed which utilizes the jet injection and the magnetic transfection. The jet injection is a method to inject medical liquid by momentary high pressure without needle. The injected liquid diffuses in the bio tissue and the endocytosis is considered to be improved by the diffusion. The magnetic transfection is a method to deliver the conjugates of plasmid DNA and magnetic particles to the desired site by external magnetic field. It is expected that jet injection of the conjugates causes slight membrane disruptions and the traction of the conjugates by magnetic field induces the efficient gene transfer. In conclusion, the possibility of improvement of the gene expression by the combination of jet injection and magnetic transfection was confirmed.","PeriodicalId":20758,"journal":{"name":"Progress in Superconductivity and Cryogenics","volume":"19 1","pages":"9-12"},"PeriodicalIF":0.2000,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Fundamental study on gene transfer utilizing magnetic force and jet injector\",\"authors\":\"T. Hasegawa, H. Nakagami, Y. Akiyama, S. Nishijima\",\"doi\":\"10.9714/PSAC.2017.19.1.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Recently, DNA vaccination is attracting attentions as a new therapeutic method for lifestyle diseases and autoimmune diseases. However, its clinical applications are limited because a safe and efficient gene transfer method has not been established yet. In this study, a new method of gene transfer was proposed which utilizes the jet injection and the magnetic transfection. The jet injection is a method to inject medical liquid by momentary high pressure without needle. The injected liquid diffuses in the bio tissue and the endocytosis is considered to be improved by the diffusion. The magnetic transfection is a method to deliver the conjugates of plasmid DNA and magnetic particles to the desired site by external magnetic field. It is expected that jet injection of the conjugates causes slight membrane disruptions and the traction of the conjugates by magnetic field induces the efficient gene transfer. In conclusion, the possibility of improvement of the gene expression by the combination of jet injection and magnetic transfection was confirmed.\",\"PeriodicalId\":20758,\"journal\":{\"name\":\"Progress in Superconductivity and Cryogenics\",\"volume\":\"19 1\",\"pages\":\"9-12\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2017-03-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Progress in Superconductivity and Cryogenics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9714/PSAC.2017.19.1.009\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHYSICS, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Progress in Superconductivity and Cryogenics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9714/PSAC.2017.19.1.009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHYSICS, APPLIED","Score":null,"Total":0}

引用次数: 1

摘要

近年来，DNA疫苗作为一种治疗生活方式疾病和自身免疫性疾病的新方法备受关注。然而，由于尚未建立安全有效的基因转移方法，其临床应用受到限制。在本研究中，提出了一种新的基因转移方法，利用喷射注射和磁性转染。喷射注射是一种在没有针头的情况下通过瞬间高压注射医用液体的方法。注入的液体在生物组织中扩散，并且内吞作用被认为通过扩散而改善。磁性转染是一种通过外部磁场将质粒DNA和磁性颗粒的缀合物递送到所需位点的方法。预计偶联物的喷射注射会引起轻微的膜破坏，并且通过磁场对偶联物的牵引会诱导有效的基因转移。总之，通过射流注射和磁性转染相结合来改善基因表达的可能性得到了证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Fundamental study on gene transfer utilizing magnetic force and jet injector

Recently, DNA vaccination is attracting attentions as a new therapeutic method for lifestyle diseases and autoimmune diseases. However, its clinical applications are limited because a safe and efficient gene transfer method has not been established yet. In this study, a new method of gene transfer was proposed which utilizes the jet injection and the magnetic transfection. The jet injection is a method to inject medical liquid by momentary high pressure without needle. The injected liquid diffuses in the bio tissue and the endocytosis is considered to be improved by the diffusion. The magnetic transfection is a method to deliver the conjugates of plasmid DNA and magnetic particles to the desired site by external magnetic field. It is expected that jet injection of the conjugates causes slight membrane disruptions and the traction of the conjugates by magnetic field induces the efficient gene transfer. In conclusion, the possibility of improvement of the gene expression by the combination of jet injection and magnetic transfection was confirmed.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Progress in Superconductivity and Cryogenics PHYSICS, APPLIED-

CiteScore

0.40

自引率

33.30%

发文量

期刊介绍： Progress in Superconductivity and Cryogenics is the official publication of The Korea Institute of Applied Superconductivity and Cryogenics and the Korean Superconductivity Society. It was launched in 1999, and accepts original research articles and review papers on research on superconductivity and related fields of physics, electronic devices, materials science, large-scale applications for magnets, power and energy, and cryogenics. The Journal is published quarterly in March, June, September, and December each year. Supplemental issues are published occasionally. The official title of the journal is ''Progress in Superconductivity and Cryogenics'' and the abbreviated title is ''Prog. Supercond. Cryog.'' All submitted manuscripts are peer-reviewed by two reviewers. The text must be written in English. All the articles in this journal are KCI and SCOPUS as of 2015. The URL address of the journal is http://psac.kisac.org where full text is available. This work was supported by the Korean Federation of Science and Technology Societies grant funded by the Korea government.