印度成人重症监护室临床分离株中对碳青霉烯耐药的鲍曼不动杆菌中OXA型碳青霉烯酶的频率

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL
Shanmugapriya Seshatri, J. Charan, V. Tak, V. Nag, S. Varthya, S. Ambwani
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引用次数: 0

摘要

摘要目的  鲍曼不动杆菌是现代医疗保健中的一种高毒力细菌,具有很高的抗微生物耐药性。碳青霉烯酶的产生似乎是对碳青霉烯产生耐药性的最常见机制。由于碳青霉烯耐药性鲍曼不动杆菌在重症监护室(ICU)患者中的患病率很高,本研究旨在寻找氧西林酶(OXA)基因的频率,包括OXA-23、OXA-24、OXA-51和OXA-58。材料和方法 从入住成人ICU的患者中采集临床标本。从耐碳青霉烯的鲍曼不动杆菌中分离DNA,并使用常规聚合酶链式反应技术和凝胶电泳进行扩增以显示结果。后果 OXA-23基因频率高达87.5%,其次是OXA-51基因,频率为73.2%。所有56个分离株的OXA-24和OXA-58基因均为阴性。我们还发现OXA-23和OXA-51基因在40个(71.4%)分离株中共存。耐药基因(OXA-23和OXA-51)与临床结果之间没有显著差异。在幸存者和非幸存者之间比较OXA基因的存在之间的关系,发现这是不显著的。OXA基因的存在没有显示住院时间的显著增加。计算了急性生理学和慢性健康评估IV评分与临床结果之间的显著相关性,出院组和死亡组的比较表明了这一点。结论 通过分子方法早期检测这些耐药基因对于减少碳青霉烯耐药鲍曼不动杆菌的传播至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Frequency of OXA-Type Carbapenemases among Carbapenem-Resistant Acinetobacter baumannii in Clinical Isolates from Adult Intensive Care Unit in India
Abstract Purpose   Acinetobacter baumannii is a highly virulent bacteria in modern health care, with a high ability to acquire antimicrobial resistance. Carbapenemases production appears to be the most common mechanism involved in drug resistance to carbapenem. As the prevalence of carbapenem-resistant A. baumannii was high in intensive care unit (ICU) patients, this study was designed to find the frequency of oxacillinases ( OXA ) genes including OXA-23 , OXA-24 , OXA-51 , and OXA-58 . Materials and Methods  A clinical specimen was collected from patients admitted to the adult ICU. DNA was isolated from carbapenem-resistant A. baumannii and amplified using conventional polymerase chain reaction technique and gel electrophoresis for visualization of results. Results  The frequency of the OXA-23 gene was high with 87.5%, followed by OXA-51 gene with 73.2%. All 56 isolates were negative for the OXA-24 and OXA-58 genes. We also found that both OXA-23 and OXA-51 genes coexisted in 40 (71.4%) isolates. No significant difference was found between drug-resistant genes ( OXA-23 and OXA-51 ) and clinical outcomes. The relationship between the presence of OXA gene was compared between survivors and nonsurvivors, which was found out to be nonsignificant. The presence of OXA genes showed no significant increase in the length of hospital stay. The significant association between acute physiology and chronic health evaluation IV scores and clinical outcome was calculated, and it was evident in the comparison of the discharged and died groups. Conclusion  Early detection of these drug-resistant genes by molecular methods is essential in decreasing the spread of carbapenem-resistant A. baumannii .
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来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
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审稿时长
31 weeks
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