海蓝Synadenium glacescens Pax叶和树干中生物活性化合物的结构鉴定和毒性评价。

D. Credo, F. Mabiki, F. Machumi, C. Cornett
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引用次数: 0

摘要

文章题目海苔的叶和茎材。在坦桑尼亚传统上用于治疗各种人类疾病。然而,关于负责其药理学特性的生物活性化合物的结构阐明和毒性特性的信息有限。因此,本研究旨在阐明和评估这些部位的生物活性化合物的毒性特性。生物活性化合物的分离是用柱色谱法实现的,而它们的结构是通过GC-MS和NMR数据以及与文献光谱数据的比较来确定的。采用卤虾致死试验(BSLT)方法对分离的化合物进行了毒性研究。在本研究中,柱色谱分离从叶中分离出四烷(1)、棕榈酸十六烷基酯(2)、二十烷(3)、四烷醇(4)和β-谷甾醇(5),从树干中分离出1-十六烷(6)、樟脑醇(7)、十八烷酸(8)和四烷酸(9)。足量分离的化合物(1、2、3、4、5和7)的BSLT对卤虾幼虫无毒(LC 50>100µg/ml)。除化合物5外,本研究首次从该植物中分离出其他八(8)种化合物。因此,可以合理地得出结论,青光眼是生物活性化合物的良好来源,这证明了其在治疗各种疾病方面的传统用途是合理的。此外,观察到的测试化合物的无毒作用表明是安全的,预计其传统用途没有毒性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structural Elucidation and Toxicity Evaluation of Bioactive Compounds from the Leaves and Stem woods of Synadenium glaucescens Pax.
The leaves and stem woods of Synadenium glaucescens Pax. are traditionally used for treatment of various human diseases in Tanzania. However, there is limited information on the structural elucidation and toxicity properties of bioactive compounds responsible for their pharmacological properties. Thus, this study was aimed to elucidate and evaluate toxicity properties of bioactive compounds from these parts. Separation of bioactive compounds was achieved by using column chromatographic method, whereas their structures were determined by GC-MS and NMR data and by comparing with literature spectroscopic data. Toxicity properties of isolated compounds was done by brine shrimp lethality test (BSLT) method. In this study, column chromatographic separation led to isolation of tetracosane (1), hexadecyl palmitate (2), octacosane (3), tetracosanol (4) and β-sitosterol (5) from the leaves and 1-hexacosene (6), campesterol (7), octadecanoic acid (8) and tetracosanoic acid (9) from the stem woods. BSLT of compounds isolated in sufficient amount (1, 2, 3, 4, 5 and 7) were revealed to be non-toxic to brine shrimp larvae (LC 50 >100 µg/ml). Except compound 5, this study reports the isolation of other eight (8) compounds for the first time from this plant. Therefore, it can be reasonably concluded that S. glaucescens is a good source of bioactive compounds which justify its traditional uses in treatment of various ailments. Additionally, observed non-toxic effect of the tested compounds indicates safety and it is expected that its traditional use has no toxicity effect.
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