对侧血管化淋巴结转移:一个优化的小鼠模型

G. Vottero, F. Morfoisse, Tania Durré, S. Blacher, G. Becker, M. Bahri, A. Plenevaux, A. Noel, J. Nizet
{"title":"对侧血管化淋巴结转移:一个优化的小鼠模型","authors":"G. Vottero, F. Morfoisse, Tania Durré, S. Blacher, G. Becker, M. Bahri, A. Plenevaux, A. Noel, J. Nizet","doi":"10.1055/s-0039-3400243","DOIUrl":null,"url":null,"abstract":"Abstract Background Vascularized lymph node transfer (VLNT) is one of the surgical options in the treatment of lymphedema, but its mechanism of action has not yet been firmly clarified. In the VLNT mouse models described so far, the lymph node flap is performed between two different sites in the same lymphedematous paw. In this study, we describe an optimized VLNT mouse model using the contralateral paw as donor site, thus removing the bias of transferring a lymph node already damaged by irradiation and/or surgery required to induce lymphedema. Methods A lymphedema was induced on the left posterior paw in four experimental groups of mice (n = 8). Two weeks later, group 1 was the sham one, group 2 underwent a VLNT from the right inguinal region to the left, in group 3 a vascular endothelial growth factor (VEGF)-C sponge was placed alone in the left inguinal region, and in group 4 a VEGF-C sponge was associated to the VLNT. The 32 mice were followed during 3 months. Outcomes included paws volume, skin quality, inflammation in the lymphedematous tissue, and lymphatic network density and function. Results Group 4 displayed significantly higher (p < 0.05) lymphedema regression compared with the other three groups. Conclusions This optimized mouse model of VLNT shows to be handy and effective. It could be exploited to perform further experimental studies about the influence of VLNT on lymphedema. Moreover, the local association between VLNT and biological compounds in this model allows it to be a good preclinical model to identify new potential drugs in lymphedema.","PeriodicalId":34024,"journal":{"name":"Journal of Reconstructive Microsurgery Open","volume":"04 1","pages":"e83 - e91"},"PeriodicalIF":0.0000,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0039-3400243","citationCount":"1","resultStr":"{\"title\":\"Contralateral Vascularized Lymph Node Transfer: An Optimized Mouse Model\",\"authors\":\"G. Vottero, F. Morfoisse, Tania Durré, S. Blacher, G. Becker, M. Bahri, A. Plenevaux, A. Noel, J. Nizet\",\"doi\":\"10.1055/s-0039-3400243\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Background Vascularized lymph node transfer (VLNT) is one of the surgical options in the treatment of lymphedema, but its mechanism of action has not yet been firmly clarified. In the VLNT mouse models described so far, the lymph node flap is performed between two different sites in the same lymphedematous paw. In this study, we describe an optimized VLNT mouse model using the contralateral paw as donor site, thus removing the bias of transferring a lymph node already damaged by irradiation and/or surgery required to induce lymphedema. Methods A lymphedema was induced on the left posterior paw in four experimental groups of mice (n = 8). Two weeks later, group 1 was the sham one, group 2 underwent a VLNT from the right inguinal region to the left, in group 3 a vascular endothelial growth factor (VEGF)-C sponge was placed alone in the left inguinal region, and in group 4 a VEGF-C sponge was associated to the VLNT. The 32 mice were followed during 3 months. Outcomes included paws volume, skin quality, inflammation in the lymphedematous tissue, and lymphatic network density and function. Results Group 4 displayed significantly higher (p < 0.05) lymphedema regression compared with the other three groups. Conclusions This optimized mouse model of VLNT shows to be handy and effective. It could be exploited to perform further experimental studies about the influence of VLNT on lymphedema. Moreover, the local association between VLNT and biological compounds in this model allows it to be a good preclinical model to identify new potential drugs in lymphedema.\",\"PeriodicalId\":34024,\"journal\":{\"name\":\"Journal of Reconstructive Microsurgery Open\",\"volume\":\"04 1\",\"pages\":\"e83 - e91\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1055/s-0039-3400243\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Reconstructive Microsurgery Open\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1055/s-0039-3400243\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Reconstructive Microsurgery Open","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1055/s-0039-3400243","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

血管化淋巴结转移(VLNT)是治疗淋巴水肿的手术选择之一,但其作用机制尚未明确。在目前描述的VLNT小鼠模型中,淋巴结瓣在同一淋巴水肿足的两个不同部位之间进行。在这项研究中,我们描述了一个优化的VLNT小鼠模型,使用对侧脚爪作为供体部位,从而消除了转移已经被照射和/或手术损伤的淋巴结的偏见,这些淋巴结需要诱导淋巴水肿。方法4组小鼠(n = 8)左后足诱导淋巴水肿。2周后,第1组为假组,第2组从右腹股沟区至左侧行VLNT,第3组在左侧腹股沟区单独放置血管内皮生长因子(VEGF)-C海绵,第4组VEGF-C海绵与VLNT联合。32只小鼠随访3个月。结果包括爪子体积、皮肤质量、淋巴水肿组织炎症、淋巴网络密度和功能。结果第4组淋巴水肿消退明显高于其他3组(p < 0.05)。结论该模型简便、有效。为进一步开展VLNT对淋巴水肿影响的实验研究奠定了基础。此外,该模型中VLNT与生物化合物之间的局部关联使其成为识别淋巴水肿新潜在药物的良好临床前模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Contralateral Vascularized Lymph Node Transfer: An Optimized Mouse Model
Abstract Background Vascularized lymph node transfer (VLNT) is one of the surgical options in the treatment of lymphedema, but its mechanism of action has not yet been firmly clarified. In the VLNT mouse models described so far, the lymph node flap is performed between two different sites in the same lymphedematous paw. In this study, we describe an optimized VLNT mouse model using the contralateral paw as donor site, thus removing the bias of transferring a lymph node already damaged by irradiation and/or surgery required to induce lymphedema. Methods A lymphedema was induced on the left posterior paw in four experimental groups of mice (n = 8). Two weeks later, group 1 was the sham one, group 2 underwent a VLNT from the right inguinal region to the left, in group 3 a vascular endothelial growth factor (VEGF)-C sponge was placed alone in the left inguinal region, and in group 4 a VEGF-C sponge was associated to the VLNT. The 32 mice were followed during 3 months. Outcomes included paws volume, skin quality, inflammation in the lymphedematous tissue, and lymphatic network density and function. Results Group 4 displayed significantly higher (p < 0.05) lymphedema regression compared with the other three groups. Conclusions This optimized mouse model of VLNT shows to be handy and effective. It could be exploited to perform further experimental studies about the influence of VLNT on lymphedema. Moreover, the local association between VLNT and biological compounds in this model allows it to be a good preclinical model to identify new potential drugs in lymphedema.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
6
审稿时长
14 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信