An integrative approach for compressive quality control of RespireAidTM, a traditional Chinese medicine formula against SARS-CoV-2

RespireAid ™ (NRICM101) is an effective anti-SARS-CoV-2 traditional Chinese medicine formula and has been licensed as a drug or dietary supplement in Taiwan, Luxembourg, Australia, Singapore, Cambodia, Philippines, and Canada. In this study, we provided integrated quality control strategy to analyze the ingredient of RespireAid ™ . In addition, the lot-to-lot ef fi cacy stabilities were also evaluated. We found that RespireAid ™ comprised of mono-saccharides and disaccharides (34.0%), maltodextrin (23.5%), inorganic elements and ash (12.2%), oligosaccharides and polysaccharides (11.4%), principal components (4.4%), moisture (4.0%), amino acids (3.5%), b -Cyclodextrin (0.25%), menthol (0.25%), and nucleotides (0.14%), while the remainder was unidenti fi ed (6.36%). This is the fi rst time that the chemical composition of a complex traditional Chinese medicine was clari fi ed using various analytical instruments. The lot-to-lot anti-oxidation and anti-in fl ammation ef fi cacies of RespireAid ™ were consistent, with average 50% scavenging concentrations of 0.22 ± 0.02 mg/mL and 5.76 ± 0.59 mg/mL, respectively. From a comprehensive quality control strategy point of view, RespireAid ™ , designed from a traditional Chinese medicine formula, displayed high quality, trans-parency, and ef fi cacy. This integrated strategy provides a clear and reliable way to evaluate the quality of complex traditional Chinese medicines.