亚最低抑菌浓度的抗生素对铜绿假单胞菌体外粘附上皮细胞能力的影响

Pub Date : 2019-09-16 DOI:10.18054/pb.v120i2-3.6749

S. Bekic, D. Drenjančević, J. Talapko, Ivan Kotris, I. Škrlec, J. Vraneš

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引用次数: 0

摘要

背景和目的：本研究的目的是通过间接免疫荧光染色，检测亚最低抑制浓度（subMIC）的头孢他啶、环丙沙星和庆大霉素对野生型铜绿假单胞菌对水牛绿猴肾细胞系的粘附能力和形态的影响。材料和方法：分别检测抗生素MIC为1/2、1/4、1/8、1/16和1/32前后细菌粘附性的变化。结果：暴露于头孢他啶和环丙沙星的所有亚MIC后，即使仅暴露于庆大霉素的1/2 MIC，附着细菌数量也有统计学差异（p<0.05）。结论：本研究结果表明，浓度远低于抑制细菌繁殖所需浓度的抗生素可能会损害铜绿假单胞菌对上皮细胞系的粘附。

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The effect of subminimal inhibitory concentrations of antibiotics on the adherence ability of Pseudomonas aeruginosa to epithelial cells in vitro

Background and purpose: The aim of this study was to examine the influence of subminimal inhibitory concentrations (subMICs) of ceftazidime, ciprofloxacin and gentamicin on the adherence ability and morphology of wild-type Pseudomonas aeruginosa strains to the Buffalo green monkey kidney cell line, using indirect immunofluorescence staining. Materials and methods: Bacterial adherence changes were tested before and after exposure to 1/2, 1/4, 1/8, 1/16 and 1/32 MIC of antibiotics. Results: A statistical difference in the number of attached bacteria after exposure to all subMICs of ceftazidime and ciprofloxacin was observed (p<0.05), even after only 1/2 MIC of gentamicin. Conclusion: The results of this study have shown that antibiotics in much lower concentrations than those necessary for inhibition of bacterial multiplications could damage the adherence of Pseudomonas aeruginosa to the epithelial cell line.

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