番茄细菌内生菌防治黄萎病及其生物刺激优势

Q3 Agricultural and Biological Sciences
N. Zendehdel, N. Hasanzadeh, F. B. Firouzjahi, S. Naeimi
{"title":"番茄细菌内生菌防治黄萎病及其生物刺激优势","authors":"N. Zendehdel, N. Hasanzadeh, F. B. Firouzjahi, S. Naeimi","doi":"10.2478/hppj-2021-0001","DOIUrl":null,"url":null,"abstract":"Summary A total of 688 bacterial endophytes were isolated from both greenhouse and field-grown tomatoes in the Tehran and Alborz provinces, Iran in order to obtain effective bacterial endophytes against the fungus Verticillium dahliae. 128 bacterial isolates, with respect to their different phenotypic characteristics were further analysed. All bacteria with positive hypersensitivity reaction on tobacco and geranium leaves and potato soft rot were eliminated, and totally 39 isolates were selected for in vitro antagonism and greenhouse tests. The potential biocontrol isolates were evaluated using seed treatment and soil drench methods on two tomato cultivars. The results indicated that seven bacterial isolates had a high potential for the control of the fungus and reduced the severity disease to 95-98%. This reduction was coincided with an increase in some growth factors like plant dry weight, root dry weight, plant height, root length, root fresh weight and plant fresh weight ranged between 92-98%. The seven antagonists’ preliminary identification was confirmed using 16SrRNA gene sequencing analysis. The BLAST analysis was performed, and the bacteria were also identified as Bacillus pumilus (two isolates), Bacillus subtilis, Bacillus safensis, Enterobacter ludwigi, Serratia marcesens and Pseudomonas beatica. Biocontrol mechanisms examination indicated that protease production was positive for all isolates and differentiated isolates E. ludwigii and P. beatica as higher producers with protease levels up to 65%. The three bacteriocins producing isolates inhibited the phytopathogenic mycelium up to 70% in dual culture assay. Also, five of the isolates produced siderophores and P. baetica, S. marcesens and E. ludwigii produced remarkable amount of auxin hormone.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"14 1","pages":"1 - 13"},"PeriodicalIF":0.0000,"publicationDate":"2020-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Tomato bacterial endophytes in controlling Verticillium wilt and their advantage on biostimulation\",\"authors\":\"N. Zendehdel, N. Hasanzadeh, F. B. Firouzjahi, S. Naeimi\",\"doi\":\"10.2478/hppj-2021-0001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Summary A total of 688 bacterial endophytes were isolated from both greenhouse and field-grown tomatoes in the Tehran and Alborz provinces, Iran in order to obtain effective bacterial endophytes against the fungus Verticillium dahliae. 128 bacterial isolates, with respect to their different phenotypic characteristics were further analysed. All bacteria with positive hypersensitivity reaction on tobacco and geranium leaves and potato soft rot were eliminated, and totally 39 isolates were selected for in vitro antagonism and greenhouse tests. The potential biocontrol isolates were evaluated using seed treatment and soil drench methods on two tomato cultivars. The results indicated that seven bacterial isolates had a high potential for the control of the fungus and reduced the severity disease to 95-98%. This reduction was coincided with an increase in some growth factors like plant dry weight, root dry weight, plant height, root length, root fresh weight and plant fresh weight ranged between 92-98%. The seven antagonists’ preliminary identification was confirmed using 16SrRNA gene sequencing analysis. The BLAST analysis was performed, and the bacteria were also identified as Bacillus pumilus (two isolates), Bacillus subtilis, Bacillus safensis, Enterobacter ludwigi, Serratia marcesens and Pseudomonas beatica. Biocontrol mechanisms examination indicated that protease production was positive for all isolates and differentiated isolates E. ludwigii and P. beatica as higher producers with protease levels up to 65%. The three bacteriocins producing isolates inhibited the phytopathogenic mycelium up to 70% in dual culture assay. Also, five of the isolates produced siderophores and P. baetica, S. marcesens and E. ludwigii produced remarkable amount of auxin hormone.\",\"PeriodicalId\":39459,\"journal\":{\"name\":\"Hellenic Plant Protection Journal\",\"volume\":\"14 1\",\"pages\":\"1 - 13\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hellenic Plant Protection Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/hppj-2021-0001\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hellenic Plant Protection Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/hppj-2021-0001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1

摘要

摘要从伊朗德黑兰省和阿尔伯茨省的温室和田间种植的番茄中共分离出688种细菌内生菌,以获得有效的细菌内生菌株来对抗真菌大丽花黄萎菌。进一步分析了128个菌株的不同表型特征。所有对烟草、天竺葵叶和马铃薯软腐病具有阳性超敏反应的细菌均被清除,共筛选出39个菌株进行体外拮抗和温室试验。采用种子处理和土壤浸种方法对两个番茄品种的潜在生防菌进行了评价。结果表明,7株分离菌株对该真菌具有很高的控制潜力,并将疾病的严重程度降低到95-98%。这种减少与一些生长因子的增加相吻合,如植物干重、根干重、株高、根长、根鲜重和植物鲜重的增加范围在92-98%之间。使用16SrRNA基因测序分析证实了七种拮抗剂的初步鉴定。进行BLAST分析,细菌还被鉴定为短小芽孢杆菌(两个分离株)、枯草芽孢杆菌、安全芽孢杆菌、鲁氏肠杆菌、马尔森沙雷氏菌和白假单胞菌。生物防治机制研究表明,所有分离株的蛋白酶产量均为阳性,分化株E.ludwigii和P.beatica为较高的蛋白酶产量,蛋白酶水平高达65%。在双培养试验中,三个产生细菌素的菌株对植物病原菌丝体的抑制率高达70%。此外,其中5个分离株产生铁载体,P.baetica、S.maresens和E.ludwigii产生显著量的生长素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tomato bacterial endophytes in controlling Verticillium wilt and their advantage on biostimulation
Summary A total of 688 bacterial endophytes were isolated from both greenhouse and field-grown tomatoes in the Tehran and Alborz provinces, Iran in order to obtain effective bacterial endophytes against the fungus Verticillium dahliae. 128 bacterial isolates, with respect to their different phenotypic characteristics were further analysed. All bacteria with positive hypersensitivity reaction on tobacco and geranium leaves and potato soft rot were eliminated, and totally 39 isolates were selected for in vitro antagonism and greenhouse tests. The potential biocontrol isolates were evaluated using seed treatment and soil drench methods on two tomato cultivars. The results indicated that seven bacterial isolates had a high potential for the control of the fungus and reduced the severity disease to 95-98%. This reduction was coincided with an increase in some growth factors like plant dry weight, root dry weight, plant height, root length, root fresh weight and plant fresh weight ranged between 92-98%. The seven antagonists’ preliminary identification was confirmed using 16SrRNA gene sequencing analysis. The BLAST analysis was performed, and the bacteria were also identified as Bacillus pumilus (two isolates), Bacillus subtilis, Bacillus safensis, Enterobacter ludwigi, Serratia marcesens and Pseudomonas beatica. Biocontrol mechanisms examination indicated that protease production was positive for all isolates and differentiated isolates E. ludwigii and P. beatica as higher producers with protease levels up to 65%. The three bacteriocins producing isolates inhibited the phytopathogenic mycelium up to 70% in dual culture assay. Also, five of the isolates produced siderophores and P. baetica, S. marcesens and E. ludwigii produced remarkable amount of auxin hormone.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Hellenic Plant Protection Journal
Hellenic Plant Protection Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
1.50
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信