蜂花粉提取物纳米颗粒的制备和表征:它们在联合治疗人类A549肺癌细胞中的潜力

IF 4.6 Q1 CHEMISTRY, APPLIED
Nemany A.N. Hanafy , Elsayed I. Salim , Magdy E. Mahfouz , Eman A. Eltonouby , Izar H. Hamed
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引用次数: 9

摘要

本研究以牛血清白蛋白(BSA)为原料制备了基于蜂花粉提取物(BPE)的聚合物纳米颗粒(BPENP),并用叶酸偶联鱼精蛋白包被该复合物,以靶向癌细胞。采用光谱学和显微方法对合成的纳米颗粒进行了表征。HPLC法测定其黄酮和多酚含量高。在336 nm处,发现BPENP具有最高的紫外吸收。平均粒径在25 - 40纳米之间。检测获得的BPE、BPENP、阿瓦斯汀及其联合用药对A549肺癌细胞的抑制作用及IC50值(24 h和48 h)。BPENP与阿瓦斯汀联合治疗试验显示两药协同作用非常显著,联合指数(CI)较低。这些治疗特别是联合治疗显著影响了HRAS、MAPK和凋亡基因如Bax、Bcl-2和Caspase 3的表达。结果表明,获得的BPENP可用于支持阿瓦斯汀治疗肺癌的给药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fabrication and characterization of bee pollen extract nanoparticles: Their potential in combination therapy against human A549 lung cancer cells

Bee pollen extract (BPE) based polymer nanoparticles (BPENP) were fabricated in the current study by using bovine serum albumin (BSA) and the complex was then coated by folic acid conjugated protamine to be targeted for cancer cells. Spectroscopic and microscopic methods were used to characterize the resultant nanoparticles. High flavonoid and polyphenolic contents were detected by HPLC. At 336 nm, it was found that BPENP provided the highest absorption by UV. The average particle size ranged between 25 and 40 nm. The inhibitory effects and IC50 values of the acquired BPE, BPENP, Avastin, or their combination at 24 and 48 h were detected against A549 lung cancer cells. The combination therapy assay of BPENP and Avastin revealed a very significant synergism between the two drugs with a low combination index (CI). These therapies particularly the combination therapy have significantly affected the expression of the HRAS, MAPK, and apoptotic genes such as Bax, Bcl-2, and Caspase 3. It was established that obtained BPENP might be employed to support the administration of Avastin for the treatment of lung cancer.

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CiteScore
4.50
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