苏云金芽孢杆菌INTA Fr7-4质粒pFR260的全序列和结构

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology

Journal of Molecular Microbiology and Biotechnology Pub Date : 2017-01-17 DOI:10.1159/000451056

Laura E. Navas, A. Amadio, E. M. Ortiz, D. Sauka, G. Benintende, M. Berretta, R. Zandomeni

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引用次数: 6

摘要

我们报道了阿根廷苏云金芽孢杆菌菌株INTA Fr7-4中一个260,595 bp的新巨型质粒pFR260的完整序列和分析。它携带7个杀虫基因:先前报道的3个cry8拷贝，2个vip1拷贝和2个vip2拷贝。此外，它还携带一个基因，编码一种被认为是非典型的哭泣蛋白。这些基因排列在约105 kbp大小的区域中，具有致病性岛的特征，具有潜在的鞘翅类特异性杀虫剂特征。DNA链组成的不对称(由GC斜态分析确定)和Rep蛋白参与复制起始的存在表明复制的双向机制。此外，还检测到许多参与偶联的基因和CRISPR-Cas系统。pFR260序列保存在GenBank中，登录号为KX258624。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes

We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2. Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624.

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来源期刊

Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.