从一例粘膜疾病中分离的牛病毒性腹泻病毒1b亚型的特征

IF 0.7 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Revista Mexicana de Ciencias Pecuarias Pub Date : 2022-12-27 DOI:10.22319/rmcp.v14i1.6200

Roberto Navarro-López, Juan Diego Pérez-de la Rosa, Marisol Karina Rocha-Martínez, Marcela Villarreal-Silva, Mario Solís-Hernández, Eric Rojas-Torres, Ninnet Gómez-Romero

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引用次数: 1

摘要

本报告描述了一头两岁公牛的粘膜疾病致死病例。对于病原体检测，通过RT-PCR、PCR、ELISA和病毒分离对痂、全血和粪便样本进行检测。在牛病毒性腹泻病毒（BVDV）的血液样品中获得RT-PCR阳性扩增。从结痂样品中分离出的病毒证实BVDV是临床表现的病原体。随后，基于三个部分序列的系统发育分析的遗传特征显示，在分析的样本中存在BVDV亚基因型1b。由于临床表现为粘膜疾病，这些发现表明检测到BVDV持续感染（PI）公牛；因此，这些结果证明了建立BVDV控制计划的重要性，该计划依赖于检测墨西哥牛中PI的存在。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Caracterización del virus de la diarrea viral bovino subtipo 1b aislado de un caso de la enfermedad de las mucosas

This report describes a fatal case of mucosal disease in a two-year-old bull. For causal agent detection, scab, whole blood, and feces samples were tested by RT-PCR, PCR, ELISA, and viral isolation. RT-PCR positive amplification was obtained in blood samples for bovine viral diarrhea virus (BVDV). Viral isolation from the scab samples confirmed BVDV as the causative agent of the clinical manifestations. Subsequently, genetic characterization based on phylogenetic analysis of three partial sequences revealed the presence of BVDV subgenotype 1b in analyzed samples. Due to the development of clinical manifestation named mucosal disease, these findings suggest the detection of BVDV persistently infected (PI) bull; therefore, these results demonstrate the importance of establishing BVDV control programs that rely on testing the presence of PI in cattle from Mexico.

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来源期刊

Revista Mexicana de Ciencias Pecuarias AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The MEXICAN MAGAZINE OF SCIENCES PECUARIAS is an organ of scientific and technical diffusion of the livestock sector. Its periodicity is quarterly and arbitrated by pairs in the double-blind mode. Its objective is to make known the results of the research carried out by any scientific institution, in Mexico and in any part of the world, related to the livestock sciences, particularly those that refer to the different disciplines of Veterinary Medicine and Animal Science. The Journal is bilingual, publishes the complete articles in Spanish or English and is included in various indexing services and international dissemination platforms, such as the Index of Mexican Journals of Scientific and Technological Research of the National Council of Science and Technology (CONACYT); In the EBSCO Host database; In the Network of Scientific Journals of Latin America and the Caribbean, Spain and Portugal (RedALyC); In the Ibero-American Network of Scientific Journals of Free Access Veterinary Medicine. Indexed in the ISI Journal Citation Report Science Edition; And Elsevier''s SCOPUS and EMBASE indices.