湘西牛和安格斯牛卵巢转录组的特征及比较分析

IF 0.7 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY
Deng Huifen
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引用次数: 1

摘要

湘西牛卵巢发育的调控机制尚不清楚,但卵巢的生长发育是一个复杂的过程,在生殖中起着至关重要的作用。本研究基于8月龄湘西牛和安格斯牛组织的RNA测序(RNA- seq)进行转录组分析,以确定哪些基因存在差异表达,并研究其时间表达谱。结果显示,共有165个差异基因被标注,其中58个在GO数据库中标注,73个在KEGG数据库中标注。共获得了12条较为集中的信号通路。其中,破骨细胞分化信号通路(ko04380)是参与最多的差异表达基因。其他信号通路有安非他命成瘾、单纯疱疹感染、咖啡因代谢、可卡因成瘾、百日咳、ecm受体相互作用、toll样受体信号通路、鞘糖脂生物合成-神经节系列。功能分析还表明,差异表达基因参与了京都基因与基因组百科全书(KEGG)的多种途径。GO注释表明,与生物过程和分子事件相关的基因显着活跃。KEGG通路分析显示,破骨细胞分化信号通路在湘西卵巢转录组数据中,位于ecm受体相互作用通路末端。此外,尽管与生殖功能没有明显的关联,但一些新的通路显著富集。本研究结果为进一步研究湘西牛卵巢的一般分子机制提供了分子资源,并为全面认识地方牛品种繁殖的特殊性提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Characterization and Comparative Analysis of Ovary Transcriptome in Locally Adopted Xiangxi and Angus Cattle
The regulatory mechanisms controlling the ovary development in the Xiangxi cattle are still largely unknown, yet the growth and development of ovary is a complex process that plays a crucial role in reproduction. In this study, we performed a transcriptome analysis based on the RNA sequencing (RNA-Seq) of Xiangxi cattle and Angus cattle tissue obtained from 8 months of age to identify which genes are differentially expressed and to investigate their temporal expression profiles. The results showed that a total of 165 differential genes were annotated, of which 58 were annotated in the GO database and 73 were annotated in the KEGG database. A total of 12 comparatively concentrated signal pathways were obtained. Among them, the osteoclast differentiation signaling pathway (ko04380) was the most involved in differentially expressed genes. The other signaling pathways were amphetamine addiction, herpes simplex infection, caffeine metabolism, cocaine addiction, pertussis, ECM-receptor interaction, toll-like receptor signaling pathway, and glycosphingolipid biosynthesis - ganglio series. Functional analysis also showed that the differentially expressed genes were involved in various Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. GO annotations indicated that the genes related to biological processes, and molecular events were significantly active. KEGG pathway analysis showed that the osteoclast differentiation signaling pathway in Xiangxi ovary transcriptome data, at the end ECM–receptor interaction pathways. Moreover, several novel pathways, were significantly enriched despite having no evident associations with the reproductive function. Our findings provide a molecular resource for further investigation of the general molecular mechanism of Xiangxi cattle ovary and offer new insights to understand comprehensively the specificity of local cattle breeds reproduction.
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来源期刊
Pakistan Journal of Agricultural Sciences
Pakistan Journal of Agricultural Sciences AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
1.80
自引率
25.00%
发文量
18
审稿时长
6-12 weeks
期刊介绍: Pakistan Journal of Agricultural Sciences is published in English four times a year. The journal publishes original articles on all aspects of agriculture and allied fields.
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