Nada A. El-Taweel, Mervat Sh. Sadek, Noha S. Khalifa
{"title":"脱落酸对豌豆生长速率、种子发育和蛋白质谱的影响。","authors":"Nada A. El-Taweel, Mervat Sh. Sadek, Noha S. Khalifa","doi":"10.21608/ejbo.2023.202685.2295","DOIUrl":null,"url":null,"abstract":"I N THIS work, cultivated pea ( Pisum sativum L. cv. Master B) is used as a legume model crop plant to investigate the effect of abscisic acid (ABA) treatment on seed filling during embryogenesis. This was evaluated by using two exogenously applied concentrations (10& 20 µM) during three stages of seed developments (early, intermediate and late). Phenotypic characterization of the treated plants included measurement of seed / legume length and diameter at full maturation stages after treatment. The expression of major proteins of the treated plants was detected using SDS-PAGE electrophoresis technique. Our data showed a positive effect of ABA when applied at the late stage of seed development. ABA significantly increased legume size and elevated the expression of some major proteins such as convicilin (70-75kDa), vicilin (50, 34kDa) and legumin (40kDa). The impact of these results is further discussed.","PeriodicalId":45102,"journal":{"name":"Egyptian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in Pisum sativum L.\",\"authors\":\"Nada A. El-Taweel, Mervat Sh. Sadek, Noha S. Khalifa\",\"doi\":\"10.21608/ejbo.2023.202685.2295\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"I N THIS work, cultivated pea ( Pisum sativum L. cv. Master B) is used as a legume model crop plant to investigate the effect of abscisic acid (ABA) treatment on seed filling during embryogenesis. This was evaluated by using two exogenously applied concentrations (10& 20 µM) during three stages of seed developments (early, intermediate and late). Phenotypic characterization of the treated plants included measurement of seed / legume length and diameter at full maturation stages after treatment. The expression of major proteins of the treated plants was detected using SDS-PAGE electrophoresis technique. Our data showed a positive effect of ABA when applied at the late stage of seed development. ABA significantly increased legume size and elevated the expression of some major proteins such as convicilin (70-75kDa), vicilin (50, 34kDa) and legumin (40kDa). The impact of these results is further discussed.\",\"PeriodicalId\":45102,\"journal\":{\"name\":\"Egyptian Journal of Botany\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2023-05-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Egyptian Journal of Botany\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/ejbo.2023.202685.2295\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Journal of Botany","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/ejbo.2023.202685.2295","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in Pisum sativum L.
I N THIS work, cultivated pea ( Pisum sativum L. cv. Master B) is used as a legume model crop plant to investigate the effect of abscisic acid (ABA) treatment on seed filling during embryogenesis. This was evaluated by using two exogenously applied concentrations (10& 20 µM) during three stages of seed developments (early, intermediate and late). Phenotypic characterization of the treated plants included measurement of seed / legume length and diameter at full maturation stages after treatment. The expression of major proteins of the treated plants was detected using SDS-PAGE electrophoresis technique. Our data showed a positive effect of ABA when applied at the late stage of seed development. ABA significantly increased legume size and elevated the expression of some major proteins such as convicilin (70-75kDa), vicilin (50, 34kDa) and legumin (40kDa). The impact of these results is further discussed.