胡椒通过抑制Rac1表达抑制人乳腺癌症细胞的生长和迁移

IF 1.7 4区医学 Q3 TROPICAL MEDICINE

Asian Pacific journal of tropical biomedicine Pub Date : 2022-01-01 DOI:10.4103/2221-1691.333211

Benjaporn Buranrat, Mutita Junking

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引用次数: 3

摘要

目的：研究胡椒碱对人乳腺癌症细胞的作用。方法：采用集落形成试验、伤口愈合试验、Matrigel迁移试验、流式细胞术、RT-qPCR和Western印迹试验，研究胡椒碱对人癌症细胞MCF-7和MDA-MB-231增殖和迁移的影响。结果：胡椒碱能抑制MCF-7和MDA-MB-231细胞的生长，抑制集落的形成。在乳腺癌症细胞中观察到G0/G1期的细胞减少和G2/M期的细胞阻滞。然而，这种显著作用仅在MDA-MB-231细胞中得到证实。此外，癌症细胞的迁移也受到低浓度胡椒碱的抑制。RT-qPCR和Western印迹分析显示胡椒碱下调Rac1基因和蛋白质的表达。结论：胡椒碱可通过降低Rac1基因和蛋白表达，抑制癌症细胞的生长和迁移。

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Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression

Objective: To investigate the effect of piperine on human breast cancer cells. Methods: The effect of piperine on proliferation and migration of human breast cancer cells, MCF-7 and MDA-MB-231, was investigated using colony formation assays, wound healing assays, Matrigel migration assays, flow cytometry, RT-qPCR, and Western blotting assays. Results: Piperine inhibited the growth of MCF-7 and MDA-MB-231 cells and suppressed colony formation. Cell reduction at the G0/ G1 phase and cell arrest at the G2/M phase were observed in breast cancer cells. However, the significant effect was only demonstrated in MDA-MB-231 cells. Moreover, cancer cell migration was suppressed by piperine at low concentration. RT-qPCR and Western blotting assays showed that piperine downregulated Rac1 gene and protein expression. Conclusions: Piperine could inhibit growth and migration of breast cancer cells by reducing Rac1 gene and protein expression.

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来源期刊

Asian Pacific journal of tropical biomedicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)

CiteScore

3.10

自引率

11.80%

发文量

2056

审稿时长

4 weeks

期刊介绍： The journal will cover technical and clinical studies related to health, ethical and social issues in field of biology, bacteriology, biochemistry, biotechnology, cell biology, environmental biology, microbiology, medical microbiology, pharmacology, physiology, pathology, immunology, virology, toxicology, epidemiology, vaccinology, hematology, histopathology, cytology, genetics and tropical agriculture. Articles with clinical interest and implications will be given preference.