两种不同剂量和组合冷冻保护剂对亮甲酚蓝暴露后牛卵母细胞成熟率的影响

Q3 Veterinary

American Journal of Animal and Veterinary Sciences Pub Date : 2022-02-01 DOI:10.3844/ajavsp.2022.101.107

M. Mphaphathi, H. O'Neill, T. Nedambale

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引用次数: 0

摘要

本研究的目的是阐明二甲亚砜(DMSO)、乙二醇(EG)以及两者联合使用的冷冻保护剂(CPAs)对牛卵母细胞的毒性以及亮甲酰蓝(BCB)对未成熟卵母细胞预选择的有效性。牛卵巢从当地屠宰场采集，并在屠宰后2小时内运输。卵母细胞暴露于26 mM的BCB溶液中。其他卵母细胞不暴露于BCB溶液或CPA。卵母细胞分为BCB阳性(+)和BCB阴性(−)。卵母细胞分别暴露于不同CPA浓度的DMSO、EG或DMSO + EG，结果如下:毒性鉴定发育能力卵母细胞。

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Effect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue Exposure

: The objectives of this study were to elucidate the toxicity of Dimethyl sulfoxide (DMSO), Ethylene glycol (EG), and the combination of both permitting cryoprotectants (CPAs) to cattle oocytes and the effectiveness of Brilliant cresyl blue (BCB) on immature oocytes pre-selection. Cattle ovaries were collected from the local slaughterhouse and transported within 2 hours of slaughter. The oocytes were exposed to 26 mM BCB solution. The other oocytes were not exposed to BCB solution or CPA. Oocytes were classified as BCB positive (+) or BCB negative (−). Oocytes were either exposed to DMSO, EG, or DMSO + EG at different CPA concentrations as follows: toxicity to identify developmentally competent oocytes.

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来源期刊

American Journal of Animal and Veterinary Sciences Veterinary-Veterinary (all)

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： American Journal of Animal and Veterinary Sciences, a quarterly, peer reviewed publication and is dedicated for publication of research articles in the field of biology of animals and with the scientific understanding of how animals work: from the physiology and biochemistry of tissues and major organ systems down to the structure and function of bio molecules and cells; particular emphasis would given to the studies of growth, reproduction, nutrition and lactation of farm and companion animals and how these processes may be optimized to improve animal re- productivity, health and welfare. Articles in support areas, such as genetics, soils, agricultural economics and marketing, legal aspects and the environment also are encouraged. AJAVS is an important source of researcher to study articles on protection of animal production practices, herd health and monitoring the spread of disease and prevention in both domestic and wild animals.