厌氧污泥接种生活污水微生物燃料电池的电化学监测与微生物特性研究

F. Vejarano, Neyla Benítez-Campo, E. Bravo, Oscar A. Loaiza, W. Lizcano-Valbuena
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引用次数: 5

摘要

采用双室水性阴极微生物燃料电池,对某城市污水处理厂厌氧菌的产电能力进行了研究。在100的固定负载下,电池电势与电流密度、功率密度与电流密度和电流的曲线Ω, 以监测在几个批处理循环中作为底物使用的函数的系统的电化学演变。连续运行65天后,获得了1.11µWcm-2的最大功率密度,在最后一个批次循环中,库仑效率为7%,化学需氧量去除率为76%。从阳极生物膜中厌氧培养细菌,分离出两种具有不同糖发酵能力的革兰氏阳性菌和两种革兰氏阴性菌,而对16S rRNA基因片段的分析显示,有三个克隆来自厚壁菌门、δ-变形菌门和α-变形菌。扫描电子成像分析显示,当从合成废水转变为真实废水时,产甲烷古菌的细胞多样性和增殖增加。这些结果揭示了基质浓度和产甲烷微生物的存在对微生物燃料电池发电的影响,表明未来的发展可能有助于利用这项技术净化小社区的生活污水。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electrochemical Monitoring and Microbial Characterization of a Domestic Wastewater-Fed Microbial Fuel Cell Inoculated with Anaerobic Sludge
A dual-chambered microbial fuel cell with aqueous cathode was operated with domestic wastewater to investigate the electrogenic ability of anaerobic bacteria from a municipal wastewater treatment plant. Curves of cell potential vs. current density, power density vs. current density and current at a fixed load of 100 Ω, were obtained daily to monitor the electrochemical evolution of the system as a function of substrate use in several batch cycles. A maximum power density of 1.11 µWcm-2 was obtained after 65 days of continuous operation and a coulombic efficiency of 7% and a chemical oxygen demand removal of 76% were found in the last batch cycle. Anaerobic culture of the bacteria from the anode biofilm resulted in the isolation of two Gram-positive and two Gram-negative bacteria with divergent sugar fermentation capabilities, while analysis of 16S rRNA gene fragments showed three clones from the phyla Firmicutes, δ-Proteobacteria and α-Proteobacteria. Scanning electron imaging analysis revealed an increase in cell diversity and proliferation of methanogenic archaea when changing from synthetic to real wastewater. These results reveal the influence of substrate concentration and presence of methanogenic microorganisms on the production of power in microbial fuel cells, suggesting that future developments could be a contribution for the use of this technology to decontaminate domestic wastewater in small communities.
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