基于环境DNA的ITS基因代谢分析钱塘江下游真核浮游动物群落特征

A. Zhang, Wang Jun, Ya-Jing Hao, Xiao Shanshi, Luo Wei, Ganxiang Wang, Zhou Zhiming
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引用次数: 1

摘要

真核微浮游生物在水体生物群落和维持水体生态系统稳定中起着重要作用。环境DNA元条形码提供了整合传统和新兴方法的机会,以发现更多的新物种,并开发分子生物指数,可以更快速,更频繁,更可靠地用于水质评估。为了检测钱塘江下游真核浮游生物的组合,开展了基于环境DNA的ITS基因元条形码技术。结果,对各种浮游植物、真菌和浮游动物进行了注释。将元条形码与形态学鉴定的群落分类群进行比较,发现了更多的真核浮游植物和浮游动物的门、纲和种。然而,两种方法鉴定出的绿藻属是最常见的组合,并发现Mesocyclops leuckarti和Acanthocyclops bicuspidatus都是该河流中优势种。桐庐、阜阳和文岩3个淡水样点浮游植物和浮游动物的读数比例随温度下降而降低。同时,对20类真菌进行了注释,首次对其群落特征进行了研究。真核微浮游生物的Chao1指数存在显著的空间差异。聚类分析和非度量多维尺度排序进一步证实,嘉绍-九月样品真核浮游生物群落组成在类水平上与其他样品差异最大。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Community Characteristics Analysis of Eukaryotic Microplankton via ITS Gene Metabarcoding Based on Environmental DNA in Lower Reaches of Qiantang River, China
Eukaryotic microplankton plays an important role in water biotic community and in maintaining the stability of water ecosystems. Environmental DNA metabarcoding provides the opportunity to integrate traditional and emerging approaches to discover more new species, and develop molecular biotic indices that can be more rapidly, frequently, and robustly used in water quality assessments. In order to examine assemblages of eukaryotic microplankton in lower reaches of Qiantang River, ITS gene metabarcoding technology based on environmental DNA was carried out. As a result, various species of phytoplankton, fungi and zooplankton were annotated on. More phylum, classes and specieses of eukaryotic phytoplankton and zooplankton were found after compared communities taxa based on metabarcoding with that obtained from morphological examination. Nevertheless, Chlorophyceae was the most common assemblage both identified by using these two methods, also Mesocyclops leuckarti and Acanthocyclops bicuspidatus were both found to be the dominant species of Cyclopoida in the river. Additionally, the reads proportions of phytoplankton and zooplankton at the three freshwater sampling sites (Tonglu, Fuyang and Wenyan) decreased as temperature drop. Meanwhile, twenty classes of fungi were annotated on, of which the community characteristic was first researched in the river. There were significant spatial differences in values of Chao1 index for eukaryotic microplankton. Cluster analysis and Non-metric multidimensional scaling ordination further confirmed that the community composition of eukaryotic microplankton at class level for Jiashao-September sample had the most dissimilarity with the others.
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