A. Zhang, Wang Jun, Ya-Jing Hao, Xiao Shanshi, Luo Wei, Ganxiang Wang, Zhou Zhiming
{"title":"基于环境DNA的ITS基因代谢分析钱塘江下游真核浮游动物群落特征","authors":"A. Zhang, Wang Jun, Ya-Jing Hao, Xiao Shanshi, Luo Wei, Ganxiang Wang, Zhou Zhiming","doi":"10.4236/OJAS.2020.112009","DOIUrl":null,"url":null,"abstract":"Eukaryotic microplankton plays an important role in water biotic community \nand in maintaining the stability of water ecosystems. Environmental DNA metabarcoding \nprovides the opportunity to integrate traditional and emerging approaches to discover \nmore new species, and develop molecular biotic indices that can be more rapidly, \nfrequently, and robustly used in water quality assessments. In order to examine \nassemblages of eukaryotic microplankton in lower reaches of Qiantang River, ITS \ngene metabarcoding technology based on environmental DNA was carried out. As a result, \nvarious species of phytoplankton, fungi and zooplankton were annotated on. More \nphylum, classes and specieses of eukaryotic phytoplankton and zooplankton were found \nafter compared communities taxa based on metabarcoding with that obtained from morphological \nexamination. Nevertheless, Chlorophyceae was the most common assemblage both identified \nby using these two methods, also Mesocyclops \nleuckarti and Acanthocyclops \nbicuspidatus were both found to be the dominant species of Cyclopoida \nin the river. Additionally, the reads proportions of phytoplankton and zooplankton \nat the three freshwater sampling sites (Tonglu, Fuyang and Wenyan) decreased as temperature \ndrop. Meanwhile, twenty classes of fungi were annotated on, of which the community \ncharacteristic was first researched in the river. There were significant spatial differences in values of Chao1 \nindex for eukaryotic microplankton. Cluster analysis and Non-metric multidimensional \nscaling ordination further confirmed that the community composition of eukaryotic \nmicroplankton at class level for Jiashao-September sample had the most dissimilarity \nwith the others.","PeriodicalId":62784,"journal":{"name":"动物科学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Community Characteristics Analysis of Eukaryotic Microplankton via ITS Gene Metabarcoding Based on Environmental DNA in Lower Reaches of Qiantang River, China\",\"authors\":\"A. Zhang, Wang Jun, Ya-Jing Hao, Xiao Shanshi, Luo Wei, Ganxiang Wang, Zhou Zhiming\",\"doi\":\"10.4236/OJAS.2020.112009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Eukaryotic microplankton plays an important role in water biotic community \\nand in maintaining the stability of water ecosystems. Environmental DNA metabarcoding \\nprovides the opportunity to integrate traditional and emerging approaches to discover \\nmore new species, and develop molecular biotic indices that can be more rapidly, \\nfrequently, and robustly used in water quality assessments. In order to examine \\nassemblages of eukaryotic microplankton in lower reaches of Qiantang River, ITS \\ngene metabarcoding technology based on environmental DNA was carried out. As a result, \\nvarious species of phytoplankton, fungi and zooplankton were annotated on. More \\nphylum, classes and specieses of eukaryotic phytoplankton and zooplankton were found \\nafter compared communities taxa based on metabarcoding with that obtained from morphological \\nexamination. Nevertheless, Chlorophyceae was the most common assemblage both identified \\nby using these two methods, also Mesocyclops \\nleuckarti and Acanthocyclops \\nbicuspidatus were both found to be the dominant species of Cyclopoida \\nin the river. Additionally, the reads proportions of phytoplankton and zooplankton \\nat the three freshwater sampling sites (Tonglu, Fuyang and Wenyan) decreased as temperature \\ndrop. Meanwhile, twenty classes of fungi were annotated on, of which the community \\ncharacteristic was first researched in the river. There were significant spatial differences in values of Chao1 \\nindex for eukaryotic microplankton. Cluster analysis and Non-metric multidimensional \\nscaling ordination further confirmed that the community composition of eukaryotic \\nmicroplankton at class level for Jiashao-September sample had the most dissimilarity \\nwith the others.\",\"PeriodicalId\":62784,\"journal\":{\"name\":\"动物科学期刊(英文)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"动物科学期刊(英文)\",\"FirstCategoryId\":\"1091\",\"ListUrlMain\":\"https://doi.org/10.4236/OJAS.2020.112009\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"动物科学期刊(英文)","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.4236/OJAS.2020.112009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Community Characteristics Analysis of Eukaryotic Microplankton via ITS Gene Metabarcoding Based on Environmental DNA in Lower Reaches of Qiantang River, China
Eukaryotic microplankton plays an important role in water biotic community
and in maintaining the stability of water ecosystems. Environmental DNA metabarcoding
provides the opportunity to integrate traditional and emerging approaches to discover
more new species, and develop molecular biotic indices that can be more rapidly,
frequently, and robustly used in water quality assessments. In order to examine
assemblages of eukaryotic microplankton in lower reaches of Qiantang River, ITS
gene metabarcoding technology based on environmental DNA was carried out. As a result,
various species of phytoplankton, fungi and zooplankton were annotated on. More
phylum, classes and specieses of eukaryotic phytoplankton and zooplankton were found
after compared communities taxa based on metabarcoding with that obtained from morphological
examination. Nevertheless, Chlorophyceae was the most common assemblage both identified
by using these two methods, also Mesocyclops
leuckarti and Acanthocyclops
bicuspidatus were both found to be the dominant species of Cyclopoida
in the river. Additionally, the reads proportions of phytoplankton and zooplankton
at the three freshwater sampling sites (Tonglu, Fuyang and Wenyan) decreased as temperature
drop. Meanwhile, twenty classes of fungi were annotated on, of which the community
characteristic was first researched in the river. There were significant spatial differences in values of Chao1
index for eukaryotic microplankton. Cluster analysis and Non-metric multidimensional
scaling ordination further confirmed that the community composition of eukaryotic
microplankton at class level for Jiashao-September sample had the most dissimilarity
with the others.