电压依赖性钾通道的抑制介导cAMP增强的大鼠胰腺β细胞胰岛素分泌

IF 1.9 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Islets Pub Date : 2017-02-15 DOI:10.1080/19382014.2017.1280644

Yunfeng Liu, Xiangqin Zhong, Yaqin Ding, Lele Ren, T. Bai, Mengmeng Liu, Zhihong Liu, Yangyan Guo, Qing Guo, Yu Zhang, Jing Yang, Yi Zhang

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引用次数: 6

摘要

胰岛素分泌对于维持葡萄糖稳态至关重要。cAMP是调节胰岛素分泌的重要细胞内信号。在本报告中，我们发现腺苷酸环化酶(AC)激活剂forskolin或cAMP类似物db-cAMP诱导的cAMP升高不仅能增强胰岛素分泌，还能抑制Kv通道，而这些作用被AC抑制剂SQ22536逆转。cAMP介导的Kv通道抑制导致动作电位持续时间延长，部分原因是cAMP信号激活导致细胞内Ca2+升高。综上所述，结果表明Kv通道参与了camp增强的胰腺β细胞胰岛素分泌。

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Inhibition of voltage-dependent potassium channels mediates cAMP-potentiated insulin secretion in rat pancreatic β cells

ABSTRACT Insulin secretion is essential for maintenance of glucose homeostasis. An important intracellular signal regulating insulin secretion is cAMP. In this report, we showed that an increase of cAMP induced by adenylyl cyclase (AC) activator forskolin or by cAMP analog db-cAMP not only potentiated insulin secretion but also inhibited Kv channels, and these effects were reversed by AC inhibitor SQ22536. The cAMP-mediated Kv channel inhibition resulted in prolongation of action potential duration, which partly accounts for the elevation of intracellular Ca2+ induced by activation of cAMP signaling. Taken together, the results suggest that Kv channels are involved in cAMP-potentiated insulin secretion in pancreatic β cells.

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来源期刊

Islets ENDOCRINOLOGY & METABOLISM-

CiteScore

3.30

自引率

4.50%

发文量

审稿时长

>12 weeks

期刊介绍： Islets is the first international, peer-reviewed research journal dedicated to islet biology. Islets publishes high-quality clinical and experimental research into the physiology and pathology of the islets of Langerhans. In addition to original research manuscripts, Islets is the leading source for cutting-edge Perspectives, Reviews and Commentaries. Our goal is to foster communication and a rapid exchange of information through timely publication of important results using print as well as electronic formats.