Alexey S. Bogdanov, Lyudmila A. Khlyap, Haluk Kefelioğlu, Ahmet Y. Selçuk, Valery V. Stakheev, Marina I. Baskevich
{"title":"土田鼠亚属三种松田鼠的高分子变异及其可能的多态性来源","authors":"Alexey S. Bogdanov, Lyudmila A. Khlyap, Haluk Kefelioğlu, Ahmet Y. Selçuk, Valery V. Stakheev, Marina I. Baskevich","doi":"10.1111/jzs.12539","DOIUrl":null,"url":null,"abstract":"<p>We studied molecular variability to clarify intraspecific differentiation and phylogenetic relationships in three pine vole species (genus <i>Microtus</i>, subgenus <i>Terricola</i>): <i>Microtus subterraneus</i>, <i>Microtus daghestanicus</i>, and <i>Microtus majori</i>. Multilocus analysis was performed using the entire mitochondrial <i>cytb</i> gene and fragments of nuclear <i>BRCA1</i>, <i>IRBP</i>, and <i>XIST</i> genes. Results confirmed separation of the species, especially <i>M</i>. <i>majori</i> compared with <i>M</i>. <i>daghestanicus</i> and <i>M</i>. <i>subterraneus</i>. These species showed different molecular polymorphism in the genetic markers. We identified two close forms of <i>M</i>. <i>majori</i>, differing in <i>cytb</i> gene and the nuclear gene <i>XIST</i>; one form inhabits the northern slopes of the Greater Caucasus, another the Transcaucasia. Separation of <i>M</i>. <i>daghestanicus</i> populations from North Ossetia and the others was clear. <i>Microtus subterraneus</i> populations from southern Europe and Asia Minor were characterized by maximal genetic heterogeneity; the specimen from Samsun (northern Asia Minor) appeared to be most distant from the others. Despite polymorphism in the chromosome number in <i>M</i>. <i>subterraneus</i> populations from the East European Plain, they possess a depleted gene pool. Results indicated that <i>M</i>. <i>subterraneus</i> colonized the East European Plain in the Holocene, and chromosome variability originated in this part of the species' range as a result of chromosomal fission and quick fixation of the arrangement in northern populations. We argue that differences in the genetic differentiation patterns of <i>Terricola</i> species are mainly due to their ecological peculiarities.</p>","PeriodicalId":54751,"journal":{"name":"Journal of Zoological Systematics and Evolutionary Research","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2021-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"High molecular variability in three pine vole species of the subgenus Terricola (Microtus, Arvicolinae) and plausible source of polymorphism\",\"authors\":\"Alexey S. Bogdanov, Lyudmila A. Khlyap, Haluk Kefelioğlu, Ahmet Y. Selçuk, Valery V. Stakheev, Marina I. Baskevich\",\"doi\":\"10.1111/jzs.12539\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>We studied molecular variability to clarify intraspecific differentiation and phylogenetic relationships in three pine vole species (genus <i>Microtus</i>, subgenus <i>Terricola</i>): <i>Microtus subterraneus</i>, <i>Microtus daghestanicus</i>, and <i>Microtus majori</i>. Multilocus analysis was performed using the entire mitochondrial <i>cytb</i> gene and fragments of nuclear <i>BRCA1</i>, <i>IRBP</i>, and <i>XIST</i> genes. Results confirmed separation of the species, especially <i>M</i>. <i>majori</i> compared with <i>M</i>. <i>daghestanicus</i> and <i>M</i>. <i>subterraneus</i>. These species showed different molecular polymorphism in the genetic markers. We identified two close forms of <i>M</i>. <i>majori</i>, differing in <i>cytb</i> gene and the nuclear gene <i>XIST</i>; one form inhabits the northern slopes of the Greater Caucasus, another the Transcaucasia. Separation of <i>M</i>. <i>daghestanicus</i> populations from North Ossetia and the others was clear. <i>Microtus subterraneus</i> populations from southern Europe and Asia Minor were characterized by maximal genetic heterogeneity; the specimen from Samsun (northern Asia Minor) appeared to be most distant from the others. Despite polymorphism in the chromosome number in <i>M</i>. <i>subterraneus</i> populations from the East European Plain, they possess a depleted gene pool. Results indicated that <i>M</i>. <i>subterraneus</i> colonized the East European Plain in the Holocene, and chromosome variability originated in this part of the species' range as a result of chromosomal fission and quick fixation of the arrangement in northern populations. We argue that differences in the genetic differentiation patterns of <i>Terricola</i> species are mainly due to their ecological peculiarities.</p>\",\"PeriodicalId\":54751,\"journal\":{\"name\":\"Journal of Zoological Systematics and Evolutionary Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2021-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Zoological Systematics and Evolutionary Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jzs.12539\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"EVOLUTIONARY BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Zoological Systematics and Evolutionary Research","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jzs.12539","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"EVOLUTIONARY BIOLOGY","Score":null,"Total":0}
High molecular variability in three pine vole species of the subgenus Terricola (Microtus, Arvicolinae) and plausible source of polymorphism
We studied molecular variability to clarify intraspecific differentiation and phylogenetic relationships in three pine vole species (genus Microtus, subgenus Terricola): Microtus subterraneus, Microtus daghestanicus, and Microtus majori. Multilocus analysis was performed using the entire mitochondrial cytb gene and fragments of nuclear BRCA1, IRBP, and XIST genes. Results confirmed separation of the species, especially M. majori compared with M. daghestanicus and M. subterraneus. These species showed different molecular polymorphism in the genetic markers. We identified two close forms of M. majori, differing in cytb gene and the nuclear gene XIST; one form inhabits the northern slopes of the Greater Caucasus, another the Transcaucasia. Separation of M. daghestanicus populations from North Ossetia and the others was clear. Microtus subterraneus populations from southern Europe and Asia Minor were characterized by maximal genetic heterogeneity; the specimen from Samsun (northern Asia Minor) appeared to be most distant from the others. Despite polymorphism in the chromosome number in M. subterraneus populations from the East European Plain, they possess a depleted gene pool. Results indicated that M. subterraneus colonized the East European Plain in the Holocene, and chromosome variability originated in this part of the species' range as a result of chromosomal fission and quick fixation of the arrangement in northern populations. We argue that differences in the genetic differentiation patterns of Terricola species are mainly due to their ecological peculiarities.
期刊介绍:
The Journal of Zoological Systematics and Evolutionary Research (JZSER)is a peer-reviewed, international forum for publication of high-quality research on systematic zoology and evolutionary biology. The aim of the journal is to provoke a synthesis of results from morphology, physiology, animal geography, ecology, ethology, evolutionary genetics, population genetics, developmental biology and molecular biology. Besides empirical papers, theoretical contributions and review articles are welcome. Integrative and interdisciplinary contributions are particularly preferred. Purely taxonomic and predominantly cytogenetic manuscripts will not be accepted except in rare cases, and then only at the Editor-in-Chief''s discretion. The same is true for phylogenetic studies based solely on mitochondrial marker sequences without any additional methodological approach. To encourage scientific exchange and discussions, authors are invited to send critical comments on previously published articles. Only papers in English language are accepted.